Molecular mechanisms for Pb removal by Cyanidiales: a potential biomaterial applied in thermo-acidic conditions

Yen Lin Cho; Yao Chang Lee; Liang Ching Hsu; Chun Chieh Wang; Pin Chen Chen; Shao Lun Liu; Heng Yi Teah; Yu Ting Liu; Yu Min Tzou

doi:10.1016/j.cej.2020.125828

Molecular mechanisms for Pb removal by Cyanidiales: a potential biomaterial applied in thermo-acidic conditions

Yen Lin Cho, Yao Chang Lee, Liang Ching Hsu, Chun Chieh Wang, Pin Chen Chen, Shao Lun Liu, Heng Yi Teah, Yu Ting Liu^*, Yu Min Tzou

^*Corresponding author for this work

Waseda Research Institute for Science and Engineering

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Thermoacidophilic Cyanidiales are capable to survive in extreme environments (20–56 °C; pH 0.5–5.0) with concentrated metals, allowing them to serve as promising green materials applied in metal remediation. It is the first attempt to determine the capacity and related mechanisms for Pb(II) ions sorption on three Cyanidiales genera: Galdieria maximum (Gm), Cyanidioschyzon merolae (Cm), and Cyanidium caldarium (Cc) in relation to changes in Pb speciation obtained from Pb L_III-edge X-ray absorption, organic functional groups, and protein secondary structures derived from synchrotron-based Fourier-transform infrared spectroscopy. Three-dimensional images of Cyanidiales were collected using Transmission X-ray microscopy. Lead tolerance on Cyanidiales was modulated according to four mechanisms: the defense line provide by polysaccharide, the inorganic Pb-PO₄ precipitation, the organic Pb complexation concomitant with the transport to cell vacuoles, and the specific thiol-Pb chelation involved in disruption of protein secondary structures. Despite Cyanidiales generally performed all mechanisms against Pb toxicity, individual defense responses were highlighted by specific Cyanidiales species. The redistribution of Pb-polysaccharide species and inorganic Pb-PO₄ precipitates toward organic complexation promoted Pb(II) ions sorption capacity of Cyanidiales, accounting for the almost eight-time-greater sorbed Pb (298.4 mg g⁻¹) on Cc in comparison to Gm. The knowledge provided here could improve the application of the Cyanidiales in environmental remediation as an innovative green technology.

Original language	English
Article number	125828
Journal	Chemical Engineering Journal
Volume	401
DOIs	https://doi.org/10.1016/j.cej.2020.125828
Publication status	Published - 2020 Dec 1

Keywords

Cyanidiales
Lead
Protein secondary structure
Speciation
Thermoacidophilic
XAS

ASJC Scopus subject areas

Chemistry(all)
Environmental Chemistry
Chemical Engineering(all)
Industrial and Manufacturing Engineering

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10.1016/j.cej.2020.125828

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@article{2107caa735aa46cab8ff2f46bdb04d29,

title = "Molecular mechanisms for Pb removal by Cyanidiales: a potential biomaterial applied in thermo-acidic conditions",

abstract = "Thermoacidophilic Cyanidiales are capable to survive in extreme environments (20–56 °C; pH 0.5–5.0) with concentrated metals, allowing them to serve as promising green materials applied in metal remediation. It is the first attempt to determine the capacity and related mechanisms for Pb(II) ions sorption on three Cyanidiales genera: Galdieria maximum (Gm), Cyanidioschyzon merolae (Cm), and Cyanidium caldarium (Cc) in relation to changes in Pb speciation obtained from Pb LIII-edge X-ray absorption, organic functional groups, and protein secondary structures derived from synchrotron-based Fourier-transform infrared spectroscopy. Three-dimensional images of Cyanidiales were collected using Transmission X-ray microscopy. Lead tolerance on Cyanidiales was modulated according to four mechanisms: the defense line provide by polysaccharide, the inorganic Pb-PO4 precipitation, the organic Pb complexation concomitant with the transport to cell vacuoles, and the specific thiol-Pb chelation involved in disruption of protein secondary structures. Despite Cyanidiales generally performed all mechanisms against Pb toxicity, individual defense responses were highlighted by specific Cyanidiales species. The redistribution of Pb-polysaccharide species and inorganic Pb-PO4 precipitates toward organic complexation promoted Pb(II) ions sorption capacity of Cyanidiales, accounting for the almost eight-time-greater sorbed Pb (298.4 mg g−1) on Cc in comparison to Gm. The knowledge provided here could improve the application of the Cyanidiales in environmental remediation as an innovative green technology.",

keywords = "Cyanidiales, Lead, Protein secondary structure, Speciation, Thermoacidophilic, XAS",

author = "Cho, {Yen Lin} and Lee, {Yao Chang} and Hsu, {Liang Ching} and Wang, {Chun Chieh} and Chen, {Pin Chen} and Liu, {Shao Lun} and Teah, {Heng Yi} and Liu, {Yu Ting} and Tzou, {Yu Min}",

note = "Funding Information: Authors are grateful to Dr. Jyh-Fu Lee for the assistance at beamline 17C, NSRRC. This work is financially supported by Ministry of Science and Technology , ROC under the project number of 107-2313-B-005-033-MY3 and MOST 108-2628-B-005-001. This work is supported in part by Ministry of Education, Taiwan, R.O.C. under Higher Education Sprout Project. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2020",

month = dec,

day = "1",

doi = "10.1016/j.cej.2020.125828",

language = "English",

volume = "401",

journal = "Chemical Engineering Journal",

issn = "1385-8947",

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T1 - Molecular mechanisms for Pb removal by Cyanidiales

T2 - a potential biomaterial applied in thermo-acidic conditions

AU - Cho, Yen Lin

AU - Lee, Yao Chang

AU - Hsu, Liang Ching

AU - Wang, Chun Chieh

AU - Chen, Pin Chen

AU - Liu, Shao Lun

AU - Teah, Heng Yi

AU - Liu, Yu Ting

AU - Tzou, Yu Min

N1 - Funding Information: Authors are grateful to Dr. Jyh-Fu Lee for the assistance at beamline 17C, NSRRC. This work is financially supported by Ministry of Science and Technology , ROC under the project number of 107-2313-B-005-033-MY3 and MOST 108-2628-B-005-001. This work is supported in part by Ministry of Education, Taiwan, R.O.C. under Higher Education Sprout Project. Publisher Copyright: © 2020 Elsevier B.V.

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Thermoacidophilic Cyanidiales are capable to survive in extreme environments (20–56 °C; pH 0.5–5.0) with concentrated metals, allowing them to serve as promising green materials applied in metal remediation. It is the first attempt to determine the capacity and related mechanisms for Pb(II) ions sorption on three Cyanidiales genera: Galdieria maximum (Gm), Cyanidioschyzon merolae (Cm), and Cyanidium caldarium (Cc) in relation to changes in Pb speciation obtained from Pb LIII-edge X-ray absorption, organic functional groups, and protein secondary structures derived from synchrotron-based Fourier-transform infrared spectroscopy. Three-dimensional images of Cyanidiales were collected using Transmission X-ray microscopy. Lead tolerance on Cyanidiales was modulated according to four mechanisms: the defense line provide by polysaccharide, the inorganic Pb-PO4 precipitation, the organic Pb complexation concomitant with the transport to cell vacuoles, and the specific thiol-Pb chelation involved in disruption of protein secondary structures. Despite Cyanidiales generally performed all mechanisms against Pb toxicity, individual defense responses were highlighted by specific Cyanidiales species. The redistribution of Pb-polysaccharide species and inorganic Pb-PO4 precipitates toward organic complexation promoted Pb(II) ions sorption capacity of Cyanidiales, accounting for the almost eight-time-greater sorbed Pb (298.4 mg g−1) on Cc in comparison to Gm. The knowledge provided here could improve the application of the Cyanidiales in environmental remediation as an innovative green technology.

AB - Thermoacidophilic Cyanidiales are capable to survive in extreme environments (20–56 °C; pH 0.5–5.0) with concentrated metals, allowing them to serve as promising green materials applied in metal remediation. It is the first attempt to determine the capacity and related mechanisms for Pb(II) ions sorption on three Cyanidiales genera: Galdieria maximum (Gm), Cyanidioschyzon merolae (Cm), and Cyanidium caldarium (Cc) in relation to changes in Pb speciation obtained from Pb LIII-edge X-ray absorption, organic functional groups, and protein secondary structures derived from synchrotron-based Fourier-transform infrared spectroscopy. Three-dimensional images of Cyanidiales were collected using Transmission X-ray microscopy. Lead tolerance on Cyanidiales was modulated according to four mechanisms: the defense line provide by polysaccharide, the inorganic Pb-PO4 precipitation, the organic Pb complexation concomitant with the transport to cell vacuoles, and the specific thiol-Pb chelation involved in disruption of protein secondary structures. Despite Cyanidiales generally performed all mechanisms against Pb toxicity, individual defense responses were highlighted by specific Cyanidiales species. The redistribution of Pb-polysaccharide species and inorganic Pb-PO4 precipitates toward organic complexation promoted Pb(II) ions sorption capacity of Cyanidiales, accounting for the almost eight-time-greater sorbed Pb (298.4 mg g−1) on Cc in comparison to Gm. The knowledge provided here could improve the application of the Cyanidiales in environmental remediation as an innovative green technology.

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KW - Protein secondary structure

KW - Speciation

KW - Thermoacidophilic

KW - XAS

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Molecular mechanisms for Pb removal by Cyanidiales: a potential biomaterial applied in thermo-acidic conditions

Abstract

Keywords

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