TY - JOUR
T1 - Monitoring surface-assisted biomolecular assembly by means of evanescent-field-coupled waveguide-mode nanobiosensors
AU - Gopinath, Subash C.B.
AU - Awazu, Koichi
AU - Fujimaki, Makoto
AU - Sugimoto, Katsuaki
AU - Ohki, Yoshimichi
AU - Komatsubara, Tetsuro
AU - Tominaga, Junji
AU - Kumar, Penmetcha K.R.
N1 - Funding Information:
Acknowledgments Part of this work was conducted at the AIST Nano-Processing Facility, which is supported by the “Nanotechnology Support Project” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. The authors would also like to thank the Advanced Functional Materials Research Center of Shin-Etsu Chemical Co., Ltd. for supplying the samples and Dr. Fukuda of AIST for experimental support.
PY - 2009/5
Y1 - 2009/5
N2 - Biological self-assembly is a natural process that involves various biomolecules, and finding the missing partner in these interactions is crucial for a specific biological function. Previously, we showed that evanescent-field-coupled waveguide-mode sensor in conjunction with a SiO 2 waveguide, the surfaces which contain cylindrical nanometric holes produced by atomic bombardment, allowed us to detect efficiently the biomolecular interactions. In the present studies, we showed that the assembly of biomolecules can be monitored using the evanescent-field-coupled waveguide-mode biosensor and thus provide a methodology in monitoring assembly process in macromolecular machines while they are assembling.
AB - Biological self-assembly is a natural process that involves various biomolecules, and finding the missing partner in these interactions is crucial for a specific biological function. Previously, we showed that evanescent-field-coupled waveguide-mode sensor in conjunction with a SiO 2 waveguide, the surfaces which contain cylindrical nanometric holes produced by atomic bombardment, allowed us to detect efficiently the biomolecular interactions. In the present studies, we showed that the assembly of biomolecules can be monitored using the evanescent-field-coupled waveguide-mode biosensor and thus provide a methodology in monitoring assembly process in macromolecular machines while they are assembling.
KW - Aptamer
KW - Biomolecular assembly
KW - Evanescent-field-coupled waveguide
KW - Nanobiosensor
KW - SiO
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U2 - 10.1007/s00216-009-2721-0
DO - 10.1007/s00216-009-2721-0
M3 - Article
C2 - 19277611
AN - SCOPUS:67349131608
SN - 0016-1152
VL - 394
SP - 481
EP - 488
JO - Fresenius Zeitschrift fur Analytische Chemie
JF - Fresenius Zeitschrift fur Analytische Chemie
IS - 2
ER -