Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring

Yukiko Sato; Takahiro Arakawa; Tomoya Sameshima; Taro Ueno; Takashi Funatsu; Shuichi Shoji

Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring

Yukiko Sato, Takahiro Arakawa, Tomoya Sameshima, Taro Ueno, Takashi Funatsu, Shuichi Shoji

School of Fundamental Science and Engineering

Research output: Contribution to conference › Paper › peer-review

1 Citation (Scopus)

Abstract

We develop a multi-reagent high speed exchange flow system integrated with Total Internal Reflection Fluorescent Microscopy (TIRFM). The reagent switching time within 100 ms was obtained with high performance in-channel PDMS microvalves, the real time monitoring of a single protein behavior, the chaperonin GroEL - cochaperonin GroES rapid interaction, was performed. The fabricated TIRFM microflow system enables the observation of single biomolecular level very short time reactions of a few seconds.

Original language	English
Pages	603-605
Number of pages	3
Publication status	Published - 2006 Jan 1
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 2006 Nov 5 → 2006 Nov 9

Conference

Conference	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	06/11/5 → 06/11/9

Keywords

Microflow system
PDMS microvalves
Protein analysis
Real time monitoring
Single biomolecular detection

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring. / Sato, Yukiko; Arakawa, Takahiro; Sameshima, Tomoya et al.
2006. 603-605 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conference › Paper › peer-review

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abstract = "We develop a multi-reagent high speed exchange flow system integrated with Total Internal Reflection Fluorescent Microscopy (TIRFM). The reagent switching time within 100 ms was obtained with high performance in-channel PDMS microvalves, the real time monitoring of a single protein behavior, the chaperonin GroEL - cochaperonin GroES rapid interaction, was performed. The fabricated TIRFM microflow system enables the observation of single biomolecular level very short time reactions of a few seconds.",

keywords = "Microflow system, PDMS microvalves, Protein analysis, Real time monitoring, Single biomolecular detection",

author = "Yukiko Sato and Takahiro Arakawa and Tomoya Sameshima and Taro Ueno and Takashi Funatsu and Shuichi Shoji",

year = "2006",

month = jan,

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language = "English",

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T1 - Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring

AU - Sato, Yukiko

AU - Arakawa, Takahiro

AU - Sameshima, Tomoya

AU - Ueno, Taro

AU - Funatsu, Takashi

AU - Shoji, Shuichi

PY - 2006/1/1

Y1 - 2006/1/1

N2 - We develop a multi-reagent high speed exchange flow system integrated with Total Internal Reflection Fluorescent Microscopy (TIRFM). The reagent switching time within 100 ms was obtained with high performance in-channel PDMS microvalves, the real time monitoring of a single protein behavior, the chaperonin GroEL - cochaperonin GroES rapid interaction, was performed. The fabricated TIRFM microflow system enables the observation of single biomolecular level very short time reactions of a few seconds.

AB - We develop a multi-reagent high speed exchange flow system integrated with Total Internal Reflection Fluorescent Microscopy (TIRFM). The reagent switching time within 100 ms was obtained with high performance in-channel PDMS microvalves, the real time monitoring of a single protein behavior, the chaperonin GroEL - cochaperonin GroES rapid interaction, was performed. The fabricated TIRFM microflow system enables the observation of single biomolecular level very short time reactions of a few seconds.

KW - Microflow system

KW - PDMS microvalves

KW - Protein analysis

KW - Real time monitoring

KW - Single biomolecular detection

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M3 - Paper

AN - SCOPUS:84902436762

SP - 603

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T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring

Abstract

Conference

Keywords

ASJC Scopus subject areas

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