Murine retroviral vectors expressing the tax1 gene of human T-cell leukemia virus type 1

Akagi Tsuyoshi; Nyunoya Hiroshi; Shimotohno Kunitada

doi:10.1016/0378-1119(91)90207-R

Murine retroviral vectors expressing the tax1 gene of human T-cell leukemia virus type 1

Akagi Tsuyoshi, Nyunoya Hiroshi, Shimotohno Kunitada^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Murine retroviral vectors which express the tax1 gene of human T-cell leukemia virus type 1 (HTLV-1) have been constructed. A significant increase in virus titer was achieved by inserting a portion of the gag region of Moloney murine leukemia virus. Using these vectors, tax1 was stably introduced into primary human T-cells derived from peripheral blood lymphocytes. Expression of the functional tax1 in infected cells was confirmed by trans-activation of HTLV-1 long terminal repeat-directed transcription. These vectors provide a useful means of investigating the function of tax1 in natural target cells for HTLV-1.

Original language	English
Pages (from-to)	255-259
Number of pages	5
Journal	Gene
Volume	106
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(91)90207-R
Publication status	Published - 1991 Oct 15
Externally published	Yes

Keywords

Recombinant DNA
gene transfer
human retrovirus
immortalization
primary human T lymphocytes
trans-activation

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(91)90207-R

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title = "Murine retroviral vectors expressing the tax1 gene of human T-cell leukemia virus type 1",

abstract = "Murine retroviral vectors which express the tax1 gene of human T-cell leukemia virus type 1 (HTLV-1) have been constructed. A significant increase in virus titer was achieved by inserting a portion of the gag region of Moloney murine leukemia virus. Using these vectors, tax1 was stably introduced into primary human T-cells derived from peripheral blood lymphocytes. Expression of the functional tax1 in infected cells was confirmed by trans-activation of HTLV-1 long terminal repeat-directed transcription. These vectors provide a useful means of investigating the function of tax1 in natural target cells for HTLV-1.",

keywords = "Recombinant DNA, gene transfer, human retrovirus, immortalization, primary human T lymphocytes, trans-activation",

author = "Akagi Tsuyoshi and Nyunoya Hiroshi and Shimotohno Kunitada",

note = "Funding Information: We thank Y. Asano, K. Kitamura (University of Tokyo), and T. Uede (Sapporo Medical College) for supplying cell lines. This work was supported in part by Grants-in-Aid for Cancer Research and Grants-in-Aid for a Comprehensive lo-Year Strategy for Cancer Control from the Ministry of Health and Welfare of Japan.",

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doi = "10.1016/0378-1119(91)90207-R",

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T1 - Murine retroviral vectors expressing the tax1 gene of human T-cell leukemia virus type 1

AU - Tsuyoshi, Akagi

AU - Hiroshi, Nyunoya

AU - Kunitada, Shimotohno

N1 - Funding Information: We thank Y. Asano, K. Kitamura (University of Tokyo), and T. Uede (Sapporo Medical College) for supplying cell lines. This work was supported in part by Grants-in-Aid for Cancer Research and Grants-in-Aid for a Comprehensive lo-Year Strategy for Cancer Control from the Ministry of Health and Welfare of Japan.

PY - 1991/10/15

Y1 - 1991/10/15

N2 - Murine retroviral vectors which express the tax1 gene of human T-cell leukemia virus type 1 (HTLV-1) have been constructed. A significant increase in virus titer was achieved by inserting a portion of the gag region of Moloney murine leukemia virus. Using these vectors, tax1 was stably introduced into primary human T-cells derived from peripheral blood lymphocytes. Expression of the functional tax1 in infected cells was confirmed by trans-activation of HTLV-1 long terminal repeat-directed transcription. These vectors provide a useful means of investigating the function of tax1 in natural target cells for HTLV-1.

AB - Murine retroviral vectors which express the tax1 gene of human T-cell leukemia virus type 1 (HTLV-1) have been constructed. A significant increase in virus titer was achieved by inserting a portion of the gag region of Moloney murine leukemia virus. Using these vectors, tax1 was stably introduced into primary human T-cells derived from peripheral blood lymphocytes. Expression of the functional tax1 in infected cells was confirmed by trans-activation of HTLV-1 long terminal repeat-directed transcription. These vectors provide a useful means of investigating the function of tax1 in natural target cells for HTLV-1.

KW - Recombinant DNA

KW - gene transfer

KW - human retrovirus

KW - immortalization

KW - primary human T lymphocytes

KW - trans-activation

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Murine retroviral vectors expressing the tax1 gene of human T-cell leukemia virus type 1

Abstract

Keywords

ASJC Scopus subject areas

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