Mutational analysis on the S-domain of bacterial ribonuclease P ribozyme.

Terumichi Tanaka*, Yo Kikuchi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


We have prepared a temporal engineering template for Escherichia coli RNase P RNA that has additional restriction sites on the S-domain region. We have prepared several deletion variants of S-domain region. The results showed the P12 and P13 domains are necessary for efficient enzymatic reactions and the absence of P14 and P17 domains are easily rescued by the presence of high concentration of magnesium ions.

Original languageEnglish
Pages (from-to)371-372
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
Publication statusPublished - 2007
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine


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