N-acetylmannosamine improves sleep-wake quality in middle-aged mice: Relevance to autonomic nervous function

Masayoshi Kuwahara*, Koichi Ito, Koji Hayakawa, Shintaro Yagi, Kunio Shiota

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Aging is associated with a variety of physiological changes originating peripherally and centrally, including within the autonomic nervous system. Sleep-wake disturbances constitute reliable hallmarks of aging in several animal species and humans. Recent studies have been interested in N-acetylmannosamine (ManNAc) a potential therapeutic agent for improving quality of life, as well as preventing age-related cognitive decline. In this study, ManNAc (5.0. mg/ml) was administered in the drinking water of middle-aged male C57BL/6J mice (55. weeks old) for 7. days. Mice were housed under a 12:12. h light:dark cycle at 23-24. °C. We evaluated bio-behavioral activity using electrocardiogram, body temperature and locomotor activity recorded by an implanted telemetry transmitter. To estimate sleep-wake profile, surface electroencephalogram and electromyogram leads connected to a telemetry transmitter were also implanted in mice. Autonomic nervous activity was evaluated using power spectral analysis of heart rate variability. ManNAc-treated mice spent more time in a wakeful state and less time in slow wave sleep during the dark phase. Parasympathetic nervous activity was increased following ManNAc treatment, then the sympatho-vagal balance was shifted predominance of parasympathetic nervous system. Furthermore, improvement in sleep-wake pattern was associated with increased parasympathetic nervous activity. These results suggest that ManNAc treatment can improve bio-behavioral activity and sleep-wake quality in middle-aged mice. This may have implications for improving sleep patterns in elderly humans.

Original languageEnglish
Pages (from-to)56-62
Number of pages7
JournalAutonomic Neuroscience: Basic and Clinical
Publication statusPublished - 2015 Jan 1
Externally publishedYes


  • Aging
  • Autonomic nervous system
  • Body temperature
  • Heart rate
  • N-acetylmannosamine
  • Orexin
  • Sleep

ASJC Scopus subject areas

  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems
  • Medicine(all)


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