Nanofluidic channel based biosensor using Surface Enhanced Raman spectroscopy (SERS)

I. Hsien Chou*, Hope T. Beier, Maio Wang, Nan Jing, Jun Kameoka, Gerard L. Coté

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution


The Raman scattering signature of molecules has been demonstrated to be greatly enhanced, on the order of 106-1012 times, on roughened metal surfaces and clustered structures such as aggregated colloidal gold. Here we describe a method that improves reproducibility and sensitivity of the substrate for surface enhanced Raman spectroscopy (SERS) by using a nanofluidic trapping device. This nanofluidic device has a bottle neck shape composed of a microchannel leading into a nano channel that causes size-dependent trapping of nanoparticles. The analyte and Au nanoparticles, 60 nm in diameter, in aqueous solution was pumped into the channel. The nanoparticles which were larger than the narrow channel are trapped at the edge of the channel to render an enhancement of the Raman signal. We have demonstrated that the Raman scattering signal enhancement on a nanochannel-based colloidal gold cluster is able to detect 10 pM of adenine, the test analyte, without chemical modification. The efficiency and robustness of the device suggests potential for single molecule detection and multicomponent detection for biological applications and/or biotoxins.

Original languageEnglish
Title of host publicationUltrasensitive and Single-Molecule Detection Technologies II
Publication statusPublished - 2007
Externally publishedYes
EventUltrasensitive and Single-Molecule Detection Technologies II - San Jose, CA, United States
Duration: 2007 Jan 202007 Jan 23

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceUltrasensitive and Single-Molecule Detection Technologies II
Country/TerritoryUnited States
CitySan Jose, CA


  • Colloidal gold
  • Nanochannel
  • Nanofluidic device
  • Raman spectroscopy
  • SERS
  • Surface enhanced

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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