Nanofluidic devices for single molecule identification

Nan Jing; Jun Kameoka; Chin B. Su; Chao Kai Chou; Mien Chie Hung

doi:10.2494/photopolymer.21.531

Nanofluidic devices for single molecule identification

Nan Jing^*, Jun Kameoka, Chin B. Su, Chao Kai Chou, Mien Chie Hung

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

In this letter, detection of target protein molecules has been demonstrated by a two-dimensional (2D) photon burst analysis technique and microfluidic channel device. Individual molecule events are scatter plotted in terms of their photon counts and burst width in 2D photon burst analysis. Detection of target protein is verified by a higher counts-per-bin for rabbit anti-HA polyclonal antibody sample than that of the control sample from the 2D photon burst analysis. Since this is microfluidic single molecule detection scheme, it has the advantages of high sensitivity, small sample consumption and reduced processing time, comparing to the conventional immunological ensemble measurements, such as western blot, immuno-precipitation, etc.

Original language	English
Pages (from-to)	531-536
Number of pages	6
Journal	Journal of Photopolymer Science and Technology
Volume	21
Issue number	4
DOIs	https://doi.org/10.2494/photopolymer.21.531
Publication status	Published - 2008
Externally published	Yes

Keywords

Microfluidic
Quantum dot
Single molecule detection

ASJC Scopus subject areas

Polymers and Plastics
Organic Chemistry
Materials Chemistry

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10.2494/photopolymer.21.531

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title = "Nanofluidic devices for single molecule identification",

abstract = "In this letter, detection of target protein molecules has been demonstrated by a two-dimensional (2D) photon burst analysis technique and microfluidic channel device. Individual molecule events are scatter plotted in terms of their photon counts and burst width in 2D photon burst analysis. Detection of target protein is verified by a higher counts-per-bin for rabbit anti-HA polyclonal antibody sample than that of the control sample from the 2D photon burst analysis. Since this is microfluidic single molecule detection scheme, it has the advantages of high sensitivity, small sample consumption and reduced processing time, comparing to the conventional immunological ensemble measurements, such as western blot, immuno-precipitation, etc.",

keywords = "Microfluidic, Quantum dot, Single molecule detection",

author = "Nan Jing and Jun Kameoka and Su, {Chin B.} and Chou, {Chao Kai} and Hung, {Mien Chie}",

year = "2008",

doi = "10.2494/photopolymer.21.531",

language = "English",

volume = "21",

pages = "531--536",

journal = "Journal of Photopolymer Science and Technology",

issn = "0914-9244",

publisher = "Tokai University",

number = "4",

}

TY - JOUR

T1 - Nanofluidic devices for single molecule identification

AU - Jing, Nan

AU - Kameoka, Jun

AU - Su, Chin B.

AU - Chou, Chao Kai

AU - Hung, Mien Chie

PY - 2008

Y1 - 2008

N2 - In this letter, detection of target protein molecules has been demonstrated by a two-dimensional (2D) photon burst analysis technique and microfluidic channel device. Individual molecule events are scatter plotted in terms of their photon counts and burst width in 2D photon burst analysis. Detection of target protein is verified by a higher counts-per-bin for rabbit anti-HA polyclonal antibody sample than that of the control sample from the 2D photon burst analysis. Since this is microfluidic single molecule detection scheme, it has the advantages of high sensitivity, small sample consumption and reduced processing time, comparing to the conventional immunological ensemble measurements, such as western blot, immuno-precipitation, etc.

AB - In this letter, detection of target protein molecules has been demonstrated by a two-dimensional (2D) photon burst analysis technique and microfluidic channel device. Individual molecule events are scatter plotted in terms of their photon counts and burst width in 2D photon burst analysis. Detection of target protein is verified by a higher counts-per-bin for rabbit anti-HA polyclonal antibody sample than that of the control sample from the 2D photon burst analysis. Since this is microfluidic single molecule detection scheme, it has the advantages of high sensitivity, small sample consumption and reduced processing time, comparing to the conventional immunological ensemble measurements, such as western blot, immuno-precipitation, etc.

KW - Microfluidic

KW - Quantum dot

KW - Single molecule detection

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U2 - 10.2494/photopolymer.21.531

DO - 10.2494/photopolymer.21.531

M3 - Article

AN - SCOPUS:50849112435

SN - 0914-9244

VL - 21

SP - 531

EP - 536

JO - Journal of Photopolymer Science and Technology

JF - Journal of Photopolymer Science and Technology

IS - 4

ER -

Nanofluidic devices for single molecule identification

Abstract

Keywords

ASJC Scopus subject areas

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