TY - JOUR
T1 - Nitric oxide generation around buccal ganglia accompanying feeding behavior in the pond snail, Lymnaea stagnalis
AU - Kobayashi, Suguru
AU - Sadamoto, Hisayo
AU - Ogawa, Hiroto
AU - Kitamura, Yoshiichiro
AU - Oka, Kotaro
AU - Tanishita, Kazuo
AU - Ito, Etsuro
N1 - Funding Information:
This work was partly supported by a Grant-in-Aid (No. 10102001) from the Ministry of Education, Science, Sports and Culture of Japan and a grant from Narishige Zoological Science Award to E.I.
PY - 2000/9/1
Y1 - 2000/9/1
N2 - Although there are many lines of evidence for both the presence of nitric oxide synthase (NOS) in the central nervous system (CNS) and the effects of NO on activating and modulating the feeding circuit in Lymnaea stagnalis, there has been no direct evidence that NO generation in the CNS accompanies feeding behavior. In the present study, we used a NO specific electrode to measure the increase in NO concentration around the buccal ganglia when the lips of semi-intact preparations of L. stagnalis were stimulated by sucrose. The NO concentration of the buccal ganglia was significantly increased by an application of sucrose to the lips. A NO scavenger and a NOS inhibitor suppressed this increase in NO concentration. A pair of putative NO-generative neurons in the buccal ganglia, the B2 cells, are active during the inter-feeding phase, and the bursting of the B2 cell elicited by sucrose application starts simultaneously with the feeding response. The rhythmic pulses of NO generation corresponded well with the rhythmic bursting of the B2 cells, which itself corresponds to the 'fictive feeding response'. The present data provide the first direct evidence that NO is generated in the buccal ganglia of L. staganalis and is involved in a specific behavior such as feeding. (C) 2000 Elsevier Science Ireland Ltd.
AB - Although there are many lines of evidence for both the presence of nitric oxide synthase (NOS) in the central nervous system (CNS) and the effects of NO on activating and modulating the feeding circuit in Lymnaea stagnalis, there has been no direct evidence that NO generation in the CNS accompanies feeding behavior. In the present study, we used a NO specific electrode to measure the increase in NO concentration around the buccal ganglia when the lips of semi-intact preparations of L. stagnalis were stimulated by sucrose. The NO concentration of the buccal ganglia was significantly increased by an application of sucrose to the lips. A NO scavenger and a NOS inhibitor suppressed this increase in NO concentration. A pair of putative NO-generative neurons in the buccal ganglia, the B2 cells, are active during the inter-feeding phase, and the bursting of the B2 cell elicited by sucrose application starts simultaneously with the feeding response. The rhythmic pulses of NO generation corresponded well with the rhythmic bursting of the B2 cells, which itself corresponds to the 'fictive feeding response'. The present data provide the first direct evidence that NO is generated in the buccal ganglia of L. staganalis and is involved in a specific behavior such as feeding. (C) 2000 Elsevier Science Ireland Ltd.
KW - Feeding behavior
KW - Lymnaea stagnalis
KW - NO electrode
KW - Nitric Oxide
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U2 - 10.1016/S0168-0102(00)00136-X
DO - 10.1016/S0168-0102(00)00136-X
M3 - Article
C2 - 10997575
AN - SCOPUS:0034281994
SN - 0168-0102
VL - 38
SP - 27
EP - 34
JO - Neuroscience Research
JF - Neuroscience Research
IS - 1
ER -