Non-CpG methylation occurs in the regulatory region of the Sry gene

Koichiro Nishino, Naoko Hattori, Shun Sato, Yoshikazu Arai, Satoshi Tanaka, Andras Nagy, Kunio Shiota*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


The Sry (sex determining region on Y chromosome) gene is a master gene for sex determination. We previously reported that the Sry gene has tissue-dependent and differentially methylated regions (T-DMRs) by analyzing the DNA methylation states at CpG sites in the promoter regions. In this study, we found unique non-CpG methylation at the internal cytosine in the 5'-CCTGG-3' pentanucleotide sequence in the Sry T-DMR. This non-CpG methylation was detected in four mouse strains (ICR, BALB/c, DBA2 and C3H), but not in two strains (C57BL/6 and 129S1), suggesting that the CCTGG methylation is tentative and unstable. Interestingly, this CCTGG methylation was associated with demethylation of the CpG sites in the Sry T-DMR in the developmental process. A methylationmediated promoter assay showed that the CCTGG methylation promotes gene expression. Our finding shows that non-CpG methylation has unique characteristic and is still conserved in mammals.

Original languageEnglish
Pages (from-to)586-593
Number of pages8
JournalJournal of Reproduction and Development
Issue number5
Publication statusPublished - 2011 Jun 3
Externally publishedYes


  • DNA methylation
  • Non-CpG methylation
  • Sry

ASJC Scopus subject areas

  • Animal Science and Zoology


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