Non-destructive, non-contact handling method for biomaterials in micro-chamber by ultrasound

Kenji Yasuda*

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

73 Citations (Scopus)


We investigated the non-contact handling of micrometer-sized samples in the micro-chamber using acoustic radiation force for lining up the microparticles and for mixing solutions. The chamber for handling samples consists of a fused quartz cell attaching a pair of 3.5 MHz lead zirconate titanate (PZT) transducers both sides. For lining up the particles, pure water containing 7-μm polystyrene spheres was introduced into the chamber. When 3.5 MHz ultrasound was irradiated into the chamber, the particles lined up at the pressure node of ultrasound less than 0.3 μm of the spatial distribution. For mixing, samples such as erythrocytes and fluorescent dye were introduced into the chamber from the inlet which arranged at the side wall of the chamber and the laminar flow of the two different kinds of solutions keeping their boundaries is observed. When the 3.5 MHz ultrasound irradiation into the chamber started, the boundaries of the two sample's flow were broken and the erythrocytes spread and mixed into all the span of the chamber. The possible damage caused by the 3.5 MHz ultrasound during the mixing process was also measured, and no significant release of the erythrocyte's component was detected even without the degas process, which was pre-processed for preventing cavitation generation. The results suggested the potential use of acoustic radiation force for non-contact, non-destructive and time-resolved handling method for micro-chamber as the sample preparation process.

Original languageEnglish
Pages (from-to)128-135
Number of pages8
JournalSensors and Actuators, B: Chemical
Issue number1-3
Publication statusPublished - 2000 Jun 10
Externally publishedYes
EventTransducers '99 - 10th International Conference on Solid-State Sensors and Actuators - Sendai, Japan
Duration: 1999 Jun 71999 Jun 10


  • Acoustic radiation force
  • Damage
  • Line-up
  • Mixing

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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