Novel 3D coaxial flow-focusing nozzle device for the production of monodispersed collagen microspheres

Sravani Jaligama; Po Jung Huang; Jun Kameoka

doi:10.1109/EMBC.2016.7591658

Novel 3D coaxial flow-focusing nozzle device for the production of monodispersed collagen microspheres

Sravani Jaligama, Po Jung Huang, Jun Kameoka

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

We have developed a 3D coaxial flow-focusing nozzle device for the mass production of monodispersed collagen microspheres and chemically crosslinked them using EDC (1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide) and N-hydroxysuccinimide (NHS). The size of the microspheres was varied between 200 μm and 600 μm by adjusting the ratio of the flow rates of the dispersed and continuous phases. MDA231-GFP cells were attached to the surface of these particles and their viability was investigated. Because they are comprised of a natural biomaterial, these collagen microspheres will have numerous applications, including bone regeneration scaffolds for tissue engineering and analyses of cancer cell interactions in a 3D environment.

Original language	English
Title of host publication	2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	4220-4223
Number of pages	4
ISBN (Electronic)	9781457702204
DOIs	https://doi.org/10.1109/EMBC.2016.7591658
Publication status	Published - 2016 Oct 13
Externally published	Yes
Event	38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016 - Orlando, United States Duration: 2016 Aug 16 → 2016 Aug 20

Publication series

Name	Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Volume	2016-October
ISSN (Print)	1557-170X

Other

Other	38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016
Country/Territory	United States
City	Orlando
Period	16/8/16 → 16/8/20

ASJC Scopus subject areas

Signal Processing
Biomedical Engineering
Computer Vision and Pattern Recognition
Health Informatics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/EMBC.2016.7591658

Cite this

Jaligama, S., Huang, P. J., & Kameoka, J. (2016). Novel 3D coaxial flow-focusing nozzle device for the production of monodispersed collagen microspheres. In 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016 (pp. 4220-4223). Article 7591658 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS; Vol. 2016-October). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/EMBC.2016.7591658

Novel 3D coaxial flow-focusing nozzle device for the production of monodispersed collagen microspheres. / Jaligama, Sravani; Huang, Po Jung; Kameoka, Jun.
2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 4220-4223 7591658 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS; Vol. 2016-October).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Jaligama, S, Huang, PJ & Kameoka, J 2016, Novel 3D coaxial flow-focusing nozzle device for the production of monodispersed collagen microspheres. in 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016., 7591658, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS, vol. 2016-October, Institute of Electrical and Electronics Engineers Inc., pp. 4220-4223, 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016, Orlando, United States, 16/8/16. https://doi.org/10.1109/EMBC.2016.7591658

Jaligama S, Huang PJ, Kameoka J. Novel 3D coaxial flow-focusing nozzle device for the production of monodispersed collagen microspheres. In 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016. Institute of Electrical and Electronics Engineers Inc. 2016. p. 4220-4223. 7591658. (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). doi: 10.1109/EMBC.2016.7591658

Jaligama, Sravani ; Huang, Po Jung ; Kameoka, Jun. / Novel 3D coaxial flow-focusing nozzle device for the production of monodispersed collagen microspheres. 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016. Institute of Electrical and Electronics Engineers Inc., 2016. pp. 4220-4223 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

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abstract = "We have developed a 3D coaxial flow-focusing nozzle device for the mass production of monodispersed collagen microspheres and chemically crosslinked them using EDC (1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide) and N-hydroxysuccinimide (NHS). The size of the microspheres was varied between 200 μm and 600 μm by adjusting the ratio of the flow rates of the dispersed and continuous phases. MDA231-GFP cells were attached to the surface of these particles and their viability was investigated. Because they are comprised of a natural biomaterial, these collagen microspheres will have numerous applications, including bone regeneration scaffolds for tissue engineering and analyses of cancer cell interactions in a 3D environment.",

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Novel 3D coaxial flow-focusing nozzle device for the production of monodispersed collagen microspheres

Abstract

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ASJC Scopus subject areas

UN SDGs

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