TY - JOUR
T1 - Novel detection system for biomolecules using nano-sized bacterial magnetic particles and magnetic force microscopy
AU - Amemiya, Yosuke
AU - Tanaka, Tsuyoshi
AU - Yoza, Brandon
AU - Matsunaga, Tadashi
PY - 2005/11/21
Y1 - 2005/11/21
N2 - A system for streptavidin detection using biotin conjugated to nano-sized bacterial magnetic particles (BMPs) has been developed. BMPs, isolated from magnetic bacteria, were used as magnetic markers for magnetic force microscopy (MFM) imaging. The magnetic signal was obtained from a single particle using MFM without application of an external magnetic field. The number of biotin conjugated BMPs (biotin-BMPs) bound to streptavidin immobilized on the glass slides increased with streptavidin concentrations up to 100 pg/ml. The minimum streptavidin detection limit using this technique is 1 pg/ml, which is 100 times more sensitive than a conventional fluorescent detection system. This is the first report using single domain nano-sized magnetic particles as magnetic markers for biosensing. This assay system can be used for immunoassay and DNA detection with high sensitivities.
AB - A system for streptavidin detection using biotin conjugated to nano-sized bacterial magnetic particles (BMPs) has been developed. BMPs, isolated from magnetic bacteria, were used as magnetic markers for magnetic force microscopy (MFM) imaging. The magnetic signal was obtained from a single particle using MFM without application of an external magnetic field. The number of biotin conjugated BMPs (biotin-BMPs) bound to streptavidin immobilized on the glass slides increased with streptavidin concentrations up to 100 pg/ml. The minimum streptavidin detection limit using this technique is 1 pg/ml, which is 100 times more sensitive than a conventional fluorescent detection system. This is the first report using single domain nano-sized magnetic particles as magnetic markers for biosensing. This assay system can be used for immunoassay and DNA detection with high sensitivities.
KW - Bacterial magnetic particles
KW - Magnetic force microscopy
KW - Magnetosensor
KW - Streptavidin-biotin interaction
UR - http://www.scopus.com/inward/record.url?scp=27544516057&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27544516057&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2005.06.028
DO - 10.1016/j.jbiotec.2005.06.028
M3 - Article
C2 - 16111780
AN - SCOPUS:27544516057
SN - 0168-1656
VL - 120
SP - 308
EP - 314
JO - Journal of Biotechnology
JF - Journal of Biotechnology
IS - 3
ER -