One-step separation of CD20+ cells from whole blood using bacterial magnetic particles displaying protein G

Masayuki Takahashi*, Tomoko Yoshino, Haruko Takeyama, Tadashi Matsunaga

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Magnetic separation of target cells from mixtures, such as peripheral blood and bone marrow, has considerable practical potential in research and medical applications. Among the current cell separation techniques, magnetic cell separation using immunomagnetic particles has been routinely applied and has proven rapidness and simplicity. Magnetospirillum magneiicum AMB-1 synthesizes intracellular nano-sized bacterial magnetic particles (BacMPs) that are individually enveloped by a stable lipid bilayer membrane. BacMPs, which exhibit strong ferrimagnetism, can be collected easily with commercially available permanent magnets. In this study, a novel magnetic nanoparticle displaying protein G (protein G-BacMPs) was fabricated, and one-step cell separation for direct cell separation from whole blood was performed using the protein G-BacMPs. B lymphocytes (CD20+ cells), which cover less than 0.3 × 10-2 % of whole blood cells, were separated with 93% purity using protein G-BacMPs binding with anti-CD20 monoclonal antibodies. The results of this study demonstrate the utility of protein G-BacMPs and the magnetic cell separation approach based on protein G-BacMPs in numerous applications.

Original languageEnglish
Title of host publicationFrom Biological Materials to Biomimetic Material Synthesis
PublisherMaterials Research Society
Pages98-103
Number of pages6
ISBN (Print)9781605608488
DOIs
Publication statusPublished - 2008
Externally publishedYes

Publication series

NameMaterials Research Society Symposium Proceedings
Volume1094
ISSN (Print)0272-9172

ASJC Scopus subject areas

  • General Materials Science
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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