Optic nerve stimulation-induced increase of release of 3h-glutamate and 3h-aspartate but not 3h-gaba from the suprachiasmatic nucleus in slices of rat hypothalamus

Shyh Yuh Liou; Shigenobu Shibata; Katsunori Iwasaki; Showa Ueki

doi:10.1016/0361-9230(86)90182-6

Optic nerve stimulation-induced increase of release of ³h-glutamate and ³h-aspartate but not ³h-gaba from the suprachiasmatic nucleus in slices of rat hypothalamus

Shyh Yuh Liou^*, Shigenobu Shibata, Katsunori Iwasaki, Showa Ueki

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

159 Citations (Scopus)

Abstract

This study investigates the possibility of glutamate and/or aspartate as an excitatory transmitter in the retinohypothalamic fibers' terminal within the anterior hypothalamus, including the suprachiasmatic nucleus (SCN). We studied the release of [³]H-Glu and ³H-Asp from the SCN of rat hypothalamic slices produced by optic nerve stimulation. Stimulation of the optic nerve released ³H-Glu, ³H-Asp but not ³H-GABA, while stimulation of the SCN released all three. These releases were suppressed in both Ca²⁺ free Krebs and tetrodotoxin (TTX) 1 μM containing Krebs. The present result with previous electrophysiological observations strongly suggest that glutamate and/or aspartate are included in this retinohypothalamic termination, while GABA is included in intrinsic and/or extrinsic neurons of the SCN, excluding the terminal of the retinohypothalamic fibers.

Original language	English
Pages (from-to)	527-531
Number of pages	5
Journal	Brain Research Bulletin
Volume	16
Issue number	4
DOIs	https://doi.org/10.1016/0361-9230(86)90182-6
Publication status	Published - 1986 Apr
Externally published	Yes

Keywords

Electrophysiology
Excitatory amino acids
Release experiment
Retinohypothalamic pathway
Suprachiasmatic nucleus

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/0361-9230(86)90182-6

Cite this

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title = "Optic nerve stimulation-induced increase of release of 3h-glutamate and 3h-aspartate but not 3h-gaba from the suprachiasmatic nucleus in slices of rat hypothalamus",

abstract = "This study investigates the possibility of glutamate and/or aspartate as an excitatory transmitter in the retinohypothalamic fibers' terminal within the anterior hypothalamus, including the suprachiasmatic nucleus (SCN). We studied the release of [3]H-Glu and 3H-Asp from the SCN of rat hypothalamic slices produced by optic nerve stimulation. Stimulation of the optic nerve released 3H-Glu, 3H-Asp but not 3H-GABA, while stimulation of the SCN released all three. These releases were suppressed in both Ca2+ free Krebs and tetrodotoxin (TTX) 1 μM containing Krebs. The present result with previous electrophysiological observations strongly suggest that glutamate and/or aspartate are included in this retinohypothalamic termination, while GABA is included in intrinsic and/or extrinsic neurons of the SCN, excluding the terminal of the retinohypothalamic fibers.",

keywords = "Electrophysiology, Excitatory amino acids, Release experiment, Retinohypothalamic pathway, Suprachiasmatic nucleus",

author = "Liou, {Shyh Yuh} and Shigenobu Shibata and Katsunori Iwasaki and Showa Ueki",

year = "1986",

month = apr,

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language = "English",

volume = "16",

pages = "527--531",

journal = "Brain Research Bulletin",

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T1 - Optic nerve stimulation-induced increase of release of 3h-glutamate and 3h-aspartate but not 3h-gaba from the suprachiasmatic nucleus in slices of rat hypothalamus

AU - Liou, Shyh Yuh

AU - Shibata, Shigenobu

AU - Iwasaki, Katsunori

AU - Ueki, Showa

PY - 1986/4

Y1 - 1986/4

N2 - This study investigates the possibility of glutamate and/or aspartate as an excitatory transmitter in the retinohypothalamic fibers' terminal within the anterior hypothalamus, including the suprachiasmatic nucleus (SCN). We studied the release of [3]H-Glu and 3H-Asp from the SCN of rat hypothalamic slices produced by optic nerve stimulation. Stimulation of the optic nerve released 3H-Glu, 3H-Asp but not 3H-GABA, while stimulation of the SCN released all three. These releases were suppressed in both Ca2+ free Krebs and tetrodotoxin (TTX) 1 μM containing Krebs. The present result with previous electrophysiological observations strongly suggest that glutamate and/or aspartate are included in this retinohypothalamic termination, while GABA is included in intrinsic and/or extrinsic neurons of the SCN, excluding the terminal of the retinohypothalamic fibers.

AB - This study investigates the possibility of glutamate and/or aspartate as an excitatory transmitter in the retinohypothalamic fibers' terminal within the anterior hypothalamus, including the suprachiasmatic nucleus (SCN). We studied the release of [3]H-Glu and 3H-Asp from the SCN of rat hypothalamic slices produced by optic nerve stimulation. Stimulation of the optic nerve released 3H-Glu, 3H-Asp but not 3H-GABA, while stimulation of the SCN released all three. These releases were suppressed in both Ca2+ free Krebs and tetrodotoxin (TTX) 1 μM containing Krebs. The present result with previous electrophysiological observations strongly suggest that glutamate and/or aspartate are included in this retinohypothalamic termination, while GABA is included in intrinsic and/or extrinsic neurons of the SCN, excluding the terminal of the retinohypothalamic fibers.

KW - Electrophysiology

KW - Excitatory amino acids

KW - Release experiment

KW - Retinohypothalamic pathway

KW - Suprachiasmatic nucleus

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