Optical coherence microscopy of living cells and bioengineered tissue dynamics in high-resolution cross-section

Akiyuki Hasegawa, Yuji Haraguchi, Hirotoshi Oikaze, Yasuhiro Kabetani, Katsuhisa Sakaguchi, Tatsuya Shimizu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Optical coherence tomography (OCT) is a valuable tool in the cross-sectional observation/analysis of three-dimensional (3-D) biological tissues, and that histological observation is important clinically. However, the resolution of the technology is approximately 10–20 μm. In this study, optical coherence microscopy (OCM), a tomographic system combining OCT technology with a microscopic technique, was constructed for observing cells individually with a resolution at the submicrometer level. Cells and 3-D tissues fabricated by cell sheet technology were observed by OCM. Importantly, the cell nuclei and cytoplasm could be clearly distinguished, and the time-dependent dynamics of cell-sheet tissues could be observed in detail. Additionally, the 3-D migration of cells in the bioengineered tissue was also detected using OCM and metal-labeled cells. Bovine aortic endothelial cells, but not NIH3T3 murine embryonic skin fibroblasts, actively migrated within the 3-D tissues. This study showed that the OCM system would be a valuable tool in the fields of cell biology, tissue engineering, and regenerative medicine.

Original languageEnglish
Pages (from-to)481-488
Number of pages8
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number3
Publication statusPublished - 2017 Apr 1


  • cell migration
  • cell sheet
  • cross-sectional observation
  • high-resolution
  • optical coherence microscopy

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering


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