Optofluidic device for ultra-sensitive detection of proteins using surface-enhanced Raman spectroscopy

Miao Wang; Melodie Benford; Nan Jing; Gerard Coté; Jun Kameoka

doi:10.1007/s10404-008-0397-y

Optofluidic device for ultra-sensitive detection of proteins using surface-enhanced Raman spectroscopy

Miao Wang, Melodie Benford, Nan Jing, Gerard Coté, Jun Kameoka^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

An optofluidic device is reported in this paper that can highly improve the robustness of surface-enhanced Raman scattering (SERS) detection and provide fingerprint information of proteins with a concentration in the nanogram per liter range within minutes. Moreover, the conformational change of protein can also be obtained using this device. Fabricated by standard photolithography processes, the optofluidic device has a step microfluidic-nanofluidic structure, which provides robust SERS detection. The sensitivity of the device is investigated using insulin and albumin as target analytes at a concentration of 0.9 ng/L. The ability to detect conformational changes of proteins using this technology is also shown by probing these analytes before and after their denaturation.

Original language	English
Pages (from-to)	411-417
Number of pages	7
Journal	Microfluidics and Nanofluidics
Volume	6
Issue number	3
DOIs	https://doi.org/10.1007/s10404-008-0397-y
Publication status	Published - 2009
Externally published	Yes

Keywords

Conformational change
Nanofluidic
Optofluidic
Protein
SERS

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Materials Chemistry

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10.1007/s10404-008-0397-y

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title = "Optofluidic device for ultra-sensitive detection of proteins using surface-enhanced Raman spectroscopy",

abstract = "An optofluidic device is reported in this paper that can highly improve the robustness of surface-enhanced Raman scattering (SERS) detection and provide fingerprint information of proteins with a concentration in the nanogram per liter range within minutes. Moreover, the conformational change of protein can also be obtained using this device. Fabricated by standard photolithography processes, the optofluidic device has a step microfluidic-nanofluidic structure, which provides robust SERS detection. The sensitivity of the device is investigated using insulin and albumin as target analytes at a concentration of 0.9 ng/L. The ability to detect conformational changes of proteins using this technology is also shown by probing these analytes before and after their denaturation.",

keywords = "Conformational change, Nanofluidic, Optofluidic, Protein, SERS",

author = "Miao Wang and Melodie Benford and Nan Jing and Gerard Cot{\'e} and Jun Kameoka",

note = "Funding Information: Acknowledgments The fabrication work of this project was performed at the Cornell NanoScale Facility, a member of the National Nanotechnology Infrastructure Network, which is supported by the National Science Foundation (Grant ECS-0335765). The Texas A&M University Materials Characterization Facility was used to obtain the SERS spectra. The Raman spectra acquisition was supported by the National Science Foundation under Grant No. BES-0421409.",

year = "2009",

doi = "10.1007/s10404-008-0397-y",

language = "English",

volume = "6",

pages = "411--417",

journal = "Microfluidics and Nanofluidics",

issn = "1613-4982",

publisher = "Springer Verlag",

number = "3",

}

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T1 - Optofluidic device for ultra-sensitive detection of proteins using surface-enhanced Raman spectroscopy

AU - Wang, Miao

AU - Benford, Melodie

AU - Jing, Nan

AU - Coté, Gerard

AU - Kameoka, Jun

N1 - Funding Information: Acknowledgments The fabrication work of this project was performed at the Cornell NanoScale Facility, a member of the National Nanotechnology Infrastructure Network, which is supported by the National Science Foundation (Grant ECS-0335765). The Texas A&M University Materials Characterization Facility was used to obtain the SERS spectra. The Raman spectra acquisition was supported by the National Science Foundation under Grant No. BES-0421409.

PY - 2009

Y1 - 2009

N2 - An optofluidic device is reported in this paper that can highly improve the robustness of surface-enhanced Raman scattering (SERS) detection and provide fingerprint information of proteins with a concentration in the nanogram per liter range within minutes. Moreover, the conformational change of protein can also be obtained using this device. Fabricated by standard photolithography processes, the optofluidic device has a step microfluidic-nanofluidic structure, which provides robust SERS detection. The sensitivity of the device is investigated using insulin and albumin as target analytes at a concentration of 0.9 ng/L. The ability to detect conformational changes of proteins using this technology is also shown by probing these analytes before and after their denaturation.

AB - An optofluidic device is reported in this paper that can highly improve the robustness of surface-enhanced Raman scattering (SERS) detection and provide fingerprint information of proteins with a concentration in the nanogram per liter range within minutes. Moreover, the conformational change of protein can also be obtained using this device. Fabricated by standard photolithography processes, the optofluidic device has a step microfluidic-nanofluidic structure, which provides robust SERS detection. The sensitivity of the device is investigated using insulin and albumin as target analytes at a concentration of 0.9 ng/L. The ability to detect conformational changes of proteins using this technology is also shown by probing these analytes before and after their denaturation.

KW - Conformational change

KW - Nanofluidic

KW - Optofluidic

KW - Protein

KW - SERS

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SN - 1613-4982

VL - 6

SP - 411

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JO - Microfluidics and Nanofluidics

JF - Microfluidics and Nanofluidics

IS - 3

ER -

Optofluidic device for ultra-sensitive detection of proteins using surface-enhanced Raman spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

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