TY - JOUR
T1 - Origin of sex chromosomes in six groups of Rana rugosa frogs inferred from a sex-linked DNA marker
AU - Oike, Akira
AU - Watanabe, Koichiro
AU - Min, Mi Sook
AU - Tojo, Koji
AU - Kumagai, Masahide
AU - Kimoto, Yuya
AU - Yamashiro, Tadashi
AU - Matsuo, Takanori
AU - Kodama, Maho
AU - Nakamura, Yoriko
AU - Notsu, Masaru
AU - Tochimoto, Takeyoshi
AU - Fujita, Hiroyuki
AU - Ota, Maki
AU - Ito, Etsuro
AU - Yasumasu, Shigeki
AU - Nakamura, Masahisa
N1 - Funding Information:
This work was supported by Grants-in-Aid from the Ministry of Education, Science, and Culture of Japan (15K07137) to M.N. and the National Research Foundation of Korea (NRF-2012R1A1A2040065) to M-S.M.
Publisher Copyright:
© 2017 Wiley Periodicals, Inc.
PY - 2017/8
Y1 - 2017/8
N2 - Each vertebrate species, as a general rule, has either the XX/XY or ZZ/ZW chromosomes by which sex is determined. However, the Japanese Rana (R.) rugosa frog is an exception, possessing both sex-determining combinations within one species, varying with region of origin. We collected R. rugosa frogs from 104 sites around Japan and South Korea and determined the nucleotide sequences of the mitochondrial 12S ribosomal RNA gene. Based on the sequences, R. rugosa frogs were divided into four groups from Japan and one from South Korea. The ZZ/ZW type is reportedly derived from the XX/XY type, although recently a new ZZ/ZW type of R. rugosa was reported. However, it still remains unclear from where the sex chromosomes in the five groups of this species were derived. In this study, we successfully isolated a sex-linked DNA maker and used it to classify R. rugosa frogs into several groupings. From the DNA marker as well as from nucleotide analysis of the promoter region of the androgen receptor (AR) gene, we identified another female heterogametic group, designated, West-Central. The sex chromosomes in the West-Central originated from the West and Central groups. The results indicate that a sex-linked DNA marker is a verifiable tool to determine the origin of the sex chromosomes in R. rugosa frogs in which the sex-determining system has changed, during two independent events, from the male to female heterogamety.
AB - Each vertebrate species, as a general rule, has either the XX/XY or ZZ/ZW chromosomes by which sex is determined. However, the Japanese Rana (R.) rugosa frog is an exception, possessing both sex-determining combinations within one species, varying with region of origin. We collected R. rugosa frogs from 104 sites around Japan and South Korea and determined the nucleotide sequences of the mitochondrial 12S ribosomal RNA gene. Based on the sequences, R. rugosa frogs were divided into four groups from Japan and one from South Korea. The ZZ/ZW type is reportedly derived from the XX/XY type, although recently a new ZZ/ZW type of R. rugosa was reported. However, it still remains unclear from where the sex chromosomes in the five groups of this species were derived. In this study, we successfully isolated a sex-linked DNA maker and used it to classify R. rugosa frogs into several groupings. From the DNA marker as well as from nucleotide analysis of the promoter region of the androgen receptor (AR) gene, we identified another female heterogametic group, designated, West-Central. The sex chromosomes in the West-Central originated from the West and Central groups. The results indicate that a sex-linked DNA marker is a verifiable tool to determine the origin of the sex chromosomes in R. rugosa frogs in which the sex-determining system has changed, during two independent events, from the male to female heterogamety.
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U2 - 10.1002/jez.2130
DO - 10.1002/jez.2130
M3 - Article
C2 - 29356406
AN - SCOPUS:85044406204
SN - 2471-5638
VL - 327
SP - 444
EP - 452
JO - Journal of Experimental Zoology Part A: Ecological and Integrative Physiology
JF - Journal of Experimental Zoology Part A: Ecological and Integrative Physiology
IS - 7
ER -