Oxygen releasing from cellular hemoglobin

Noriyuki Kawai*, Haruki Ohkawa, Hiromitsu Maejima, Shinji Takeoka, Hiroyuki Nishide, Eishun Tsuchida

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


The oxygen-releasing behaviour of hemoglobin vesicles (HbV) was measured in order to study the difference in oxygen dynamics inside and outside the cellular Hb using a conventional stopped flow method and a newly developed stopped flow flash photolysis method. The partial pressure of oxygen in the solution outside the HbV was monitored with the lifetime of the triplet state of meso-tetraphenylporphinatozinc(II) bound to human serum albumin excited by the laser flash. The change in the partial pressure of oxygen outside the HbV showed a biphasic profile and was slower than that inside the HbV. The first phase shows the oxygen-releasing process from Hb near the phospholipid bilayer membrane, and the second phase is considered the process in which oxygen diffuses to the bulk aqueous region and reaches the equilibrium value.

Original languageEnglish
Pages (from-to)507-517
Number of pages11
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Issue number5-6
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering


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