TY - JOUR
T1 - Oxygen releasing from cellular hemoglobin
AU - Kawai, Noriyuki
AU - Ohkawa, Haruki
AU - Maejima, Hiromitsu
AU - Takeoka, Shinji
AU - Nishide, Hiroyuki
AU - Tsuchida, Eishun
N1 - Funding Information:
This work was partially supported by the project of Material Research Laboratory for BioScience and Photonics in Waseda University and Health Science Research Grants (Artificial Blood Project ) from the Ministry of Health and Welfare, Japan.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1998
Y1 - 1998
N2 - The oxygen-releasing behaviour of hemoglobin vesicles (HbV) was measured in order to study the difference in oxygen dynamics inside and outside the cellular Hb using a conventional stopped flow method and a newly developed stopped flow flash photolysis method. The partial pressure of oxygen in the solution outside the HbV was monitored with the lifetime of the triplet state of meso-tetraphenylporphinatozinc(II) bound to human serum albumin excited by the laser flash. The change in the partial pressure of oxygen outside the HbV showed a biphasic profile and was slower than that inside the HbV. The first phase shows the oxygen-releasing process from Hb near the phospholipid bilayer membrane, and the second phase is considered the process in which oxygen diffuses to the bulk aqueous region and reaches the equilibrium value.
AB - The oxygen-releasing behaviour of hemoglobin vesicles (HbV) was measured in order to study the difference in oxygen dynamics inside and outside the cellular Hb using a conventional stopped flow method and a newly developed stopped flow flash photolysis method. The partial pressure of oxygen in the solution outside the HbV was monitored with the lifetime of the triplet state of meso-tetraphenylporphinatozinc(II) bound to human serum albumin excited by the laser flash. The change in the partial pressure of oxygen outside the HbV showed a biphasic profile and was slower than that inside the HbV. The first phase shows the oxygen-releasing process from Hb near the phospholipid bilayer membrane, and the second phase is considered the process in which oxygen diffuses to the bulk aqueous region and reaches the equilibrium value.
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U2 - 10.3109/10731199809117471
DO - 10.3109/10731199809117471
M3 - Article
C2 - 9844717
AN - SCOPUS:0032211581
SN - 1073-1199
VL - 26
SP - 507
EP - 517
JO - Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
JF - Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
IS - 5-6
ER -