Abstract
We developed passive cell distributing method for cellular diagnostic microwell array. This method can distribute cells evenly and simultaneously to a number of microwells only by introducing cell suspensions from the inlet. In order to reduce the dispersion of the number of distributed cells to each microwell, we propose a novel concept of the self-regulating pinched flow. The self-regulating pinched flow is realized in a microchannel, which consists of the center micropillar colonnades and side channels. CFD simulations were carried out to optimize the design of the pinching area. Using a fabricated prototype device having 8 microwells, uniform distribution of the beads was obtained successfully. The coefficient of variation (CV) of the distributed bead in microwells was 29.3%.
| Original language | English |
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| Pages | 500-502 |
| Number of pages | 3 |
| Publication status | Published - 2006 |
| Externally published | Yes |
| Event | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 2006 Nov 5 → 2006 Nov 9 |
Conference
| Conference | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 |
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| Country/Territory | Japan |
| City | Tokyo |
| Period | 06/11/5 → 06/11/9 |
Keywords
- High throughput screening
- Microwell array
- Passive distribution
- Self-regulating pinched flow
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering