TY - GEN
T1 - PDMS-CYTOP hybrid structure microwell array chip for total internal reflection fluorescence microscopy
AU - Nakahara, A.
AU - Shirasaki, Y.
AU - Mizuno, J.
AU - Sekiguchi, T.
AU - Ohara, O.
AU - Shoji, S.
PY - 2013
Y1 - 2013
N2 - A microwell arrry device with a hybrid structure of amorphour perfluoroplymer, CYTOP™, and polydimethylsiloxane (PDMS) which is specialized in total internal refllection fluorescence (TIRF) microscopy was developed. Since the CYTOP layer, whose refractive index is as same as that of water, is sandwiched between PDMS and glass substrate, leakage of incident light is avoided. PDMS layer upon the CYTOP enables high aspect ratio structures and easy integration of microfluidic components. Since direct bonding of CYTOP and PDMS is difficult to achieve, a chemical bonding method using aminosilane coupling reagent and 3-Glycoidoxypropyltriethoxysilane was applied. The bond strength was about 0.74 MPa. 24 × 50 microwell array was fabricated and their optical properties was evaluated. Measured TIRF microscopy results shows no interfere with incident light under the conventionally used conditions.
AB - A microwell arrry device with a hybrid structure of amorphour perfluoroplymer, CYTOP™, and polydimethylsiloxane (PDMS) which is specialized in total internal refllection fluorescence (TIRF) microscopy was developed. Since the CYTOP layer, whose refractive index is as same as that of water, is sandwiched between PDMS and glass substrate, leakage of incident light is avoided. PDMS layer upon the CYTOP enables high aspect ratio structures and easy integration of microfluidic components. Since direct bonding of CYTOP and PDMS is difficult to achieve, a chemical bonding method using aminosilane coupling reagent and 3-Glycoidoxypropyltriethoxysilane was applied. The bond strength was about 0.74 MPa. 24 × 50 microwell array was fabricated and their optical properties was evaluated. Measured TIRF microscopy results shows no interfere with incident light under the conventionally used conditions.
KW - CYTOP
KW - TIRF microscopy
KW - microwell array
UR - http://www.scopus.com/inward/record.url?scp=84883066139&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883066139&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2013.6559835
DO - 10.1109/NEMS.2013.6559835
M3 - Conference contribution
AN - SCOPUS:84883066139
SN - 9781467363525
T3 - 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
SP - 743
EP - 746
BT - 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
T2 - 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
Y2 - 7 April 2013 through 10 April 2013
ER -