Abstract
We present a novel peptide-based ligand screening system for G protein-coupled receptors (GPCRs) in water-in-oil (W/O) microdroplets. This system is an in vitro gene screening system for peptides that activate a target GPCR expressed in yeast. We show that the system is applicable to human GPCRs. This system will be a powerful tool for identifying novel peptide ligands for liganded mammalian GPCRs and orphan mammalian GPCRs.
| Original language | English |
|---|---|
| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 965-967 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806476 |
| Publication status | Published - 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 2014 Oct 26 → 2014 Oct 30 |
Other
| Other | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country/Territory | United States |
| City | San Antonio |
| Period | 14/10/26 → 14/10/30 |
Keywords
- Cell-free protein synthesis
- G protein-coupled receptors
- Ligand assay
- Microdroplet
- Yeast
ASJC Scopus subject areas
- Control and Systems Engineering