Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria

Hideo Iwasaki; Yasuhito Taniguchi; Masahiro Ishiura; Takao Kondo

doi:10.1093/emboj/18.5.1137

Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria

Hideo Iwasaki, Yasuhito Taniguchi, Masahiro Ishiura, Takao Kondo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

146 Citations (Scopus)

Abstract

The kai gene cluster, which is composed of three genes, kaiA, kaiB and kaiC, is essential for the generation of circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Here we demonstrate the direct association of KaiA, KaiB and KaiC in yeast cells using the two-hybrid system, in vitro and in cyanobacterial cells. KaiC enhanced KaiA-KaiB interaction in vitro and in yeast cells, suggesting that the three Kai proteins were able to form a heteromultimeric complex. We also found that a long period mutation kaiA1 dramatically enhanced KaiA-KaiB interaction in vitro. Thus, direct protein-protein association among the Kai proteins may be a critical process in the generation of circadian rhythms in cyanobacteria.

Original language	English
Pages (from-to)	1137-1145
Number of pages	9
Journal	EMBO Journal
Volume	18
Issue number	5
DOIs	https://doi.org/10.1093/emboj/18.5.1137
Publication status	Published - 1999 Mar 1
Externally published	Yes

Keywords

Circadian rhythm
Kai genes
Protein-protein interaction
Synechococcus

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.1093/emboj/18.5.1137

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title = "Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria",

abstract = "The kai gene cluster, which is composed of three genes, kaiA, kaiB and kaiC, is essential for the generation of circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Here we demonstrate the direct association of KaiA, KaiB and KaiC in yeast cells using the two-hybrid system, in vitro and in cyanobacterial cells. KaiC enhanced KaiA-KaiB interaction in vitro and in yeast cells, suggesting that the three Kai proteins were able to form a heteromultimeric complex. We also found that a long period mutation kaiA1 dramatically enhanced KaiA-KaiB interaction in vitro. Thus, direct protein-protein association among the Kai proteins may be a critical process in the generation of circadian rhythms in cyanobacteria.",

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AU - Iwasaki, Hideo

AU - Taniguchi, Yasuhito

AU - Ishiura, Masahiro

AU - Kondo, Takao

PY - 1999/3/1

Y1 - 1999/3/1

N2 - The kai gene cluster, which is composed of three genes, kaiA, kaiB and kaiC, is essential for the generation of circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Here we demonstrate the direct association of KaiA, KaiB and KaiC in yeast cells using the two-hybrid system, in vitro and in cyanobacterial cells. KaiC enhanced KaiA-KaiB interaction in vitro and in yeast cells, suggesting that the three Kai proteins were able to form a heteromultimeric complex. We also found that a long period mutation kaiA1 dramatically enhanced KaiA-KaiB interaction in vitro. Thus, direct protein-protein association among the Kai proteins may be a critical process in the generation of circadian rhythms in cyanobacteria.

AB - The kai gene cluster, which is composed of three genes, kaiA, kaiB and kaiC, is essential for the generation of circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Here we demonstrate the direct association of KaiA, KaiB and KaiC in yeast cells using the two-hybrid system, in vitro and in cyanobacterial cells. KaiC enhanced KaiA-KaiB interaction in vitro and in yeast cells, suggesting that the three Kai proteins were able to form a heteromultimeric complex. We also found that a long period mutation kaiA1 dramatically enhanced KaiA-KaiB interaction in vitro. Thus, direct protein-protein association among the Kai proteins may be a critical process in the generation of circadian rhythms in cyanobacteria.

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KW - Kai genes

KW - Protein-protein interaction

KW - Synechococcus

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Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria

Abstract

Keywords

ASJC Scopus subject areas

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