Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays

Varun Penmatsa; A. Rahim Ruslinda; Majid Beidaghi; Hiroshi Kawarada; Chunlei Wang

doi:10.1016/j.bios.2012.06.055

Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays

Varun Penmatsa, A. Rahim Ruslinda, Majid Beidaghi, Hiroshi Kawarada, Chunlei Wang^*

^*Corresponding author for this work

School of Fundamental Science and Engineering

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

The potential of aptamers as ligand binding molecule has opened new avenues in the development of biosensors for cancer oncoproteins. In this paper, a label-free detection strategy using signaling aptamer/protein binding complex for platelet-derived growth factor (PDGF-BB) oncoprotein detection is reported. The detection mechanism is based on the release of fluorophore (TOTO intercalating dye) from the target binding aptamer's stem structure when it captures PDGF. Amino-terminated three-dimensional carbon microarrays fabricated by pyrolyzing patterned photoresist were used as a detection platform. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration even in the sub-nanomolar range with an excellent detection limit of 5. pmol. This detection strategy is promising in a wide range of applications in the detection of cancer biomarkers and other proteins.

Original language	English
Pages (from-to)	118-123
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	39
Issue number	1
DOIs	https://doi.org/10.1016/j.bios.2012.06.055
Publication status	Published - 2013 Jan 15

Keywords

3D carbon microarrays
Direct amination
Fluorescence detection
Platelet-derived growth factor

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bios.2012.06.055

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title = "Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays",

abstract = "The potential of aptamers as ligand binding molecule has opened new avenues in the development of biosensors for cancer oncoproteins. In this paper, a label-free detection strategy using signaling aptamer/protein binding complex for platelet-derived growth factor (PDGF-BB) oncoprotein detection is reported. The detection mechanism is based on the release of fluorophore (TOTO intercalating dye) from the target binding aptamer's stem structure when it captures PDGF. Amino-terminated three-dimensional carbon microarrays fabricated by pyrolyzing patterned photoresist were used as a detection platform. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration even in the sub-nanomolar range with an excellent detection limit of 5. pmol. This detection strategy is promising in a wide range of applications in the detection of cancer biomarkers and other proteins.",

keywords = "3D carbon microarrays, Direct amination, Fluorescence detection, Platelet-derived growth factor",

author = "Varun Penmatsa and Ruslinda, {A. Rahim} and Majid Beidaghi and Hiroshi Kawarada and Chunlei Wang",

note = "Funding Information: Varun Penmatsa and Majid Beidaghi gratefully acknowledges the financial support from the DEA and DYF fellowships provided by FIU graduate school . This project is partially supported by the National Science Foundation ( OISE 0934078 and CMMI 0800525 ). The authors would also like to acknowledge Grant-in-Aid from GCOE Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan and a Grant-in-Aid for Fundamental Research of Japan Society for the Promotion of Science (JSPS) ( 23246069 ). The authors would like to acknowledge the research facilities provided at AMERI at FIU and NTRC at Waseda University.",

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AU - Wang, Chunlei

N1 - Funding Information: Varun Penmatsa and Majid Beidaghi gratefully acknowledges the financial support from the DEA and DYF fellowships provided by FIU graduate school . This project is partially supported by the National Science Foundation ( OISE 0934078 and CMMI 0800525 ). The authors would also like to acknowledge Grant-in-Aid from GCOE Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan and a Grant-in-Aid for Fundamental Research of Japan Society for the Promotion of Science (JSPS) ( 23246069 ). The authors would like to acknowledge the research facilities provided at AMERI at FIU and NTRC at Waseda University.

PY - 2013/1/15

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N2 - The potential of aptamers as ligand binding molecule has opened new avenues in the development of biosensors for cancer oncoproteins. In this paper, a label-free detection strategy using signaling aptamer/protein binding complex for platelet-derived growth factor (PDGF-BB) oncoprotein detection is reported. The detection mechanism is based on the release of fluorophore (TOTO intercalating dye) from the target binding aptamer's stem structure when it captures PDGF. Amino-terminated three-dimensional carbon microarrays fabricated by pyrolyzing patterned photoresist were used as a detection platform. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration even in the sub-nanomolar range with an excellent detection limit of 5. pmol. This detection strategy is promising in a wide range of applications in the detection of cancer biomarkers and other proteins.

AB - The potential of aptamers as ligand binding molecule has opened new avenues in the development of biosensors for cancer oncoproteins. In this paper, a label-free detection strategy using signaling aptamer/protein binding complex for platelet-derived growth factor (PDGF-BB) oncoprotein detection is reported. The detection mechanism is based on the release of fluorophore (TOTO intercalating dye) from the target binding aptamer's stem structure when it captures PDGF. Amino-terminated three-dimensional carbon microarrays fabricated by pyrolyzing patterned photoresist were used as a detection platform. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration even in the sub-nanomolar range with an excellent detection limit of 5. pmol. This detection strategy is promising in a wide range of applications in the detection of cancer biomarkers and other proteins.

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Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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