Potentiometric biosensor for urea based on electropolymerized electroinactive polypyrrole

Shinichi Komaba, Michiko Seyama, Toshiyuki Momma, Tetsuya Osaka

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)


A urea biosensor was developed by immobilizing urease into an electropolymerized electroinactive polypyrrole (PPy) on a platinum electrode. This enzyme-immobilized PPy electrode showed a stable potential response to urea based on the pH response of the electroinactive PPy film electrode. The electropolymerization conditions were optimized, and thus the biosensor showed a Nernstian response with a slope of 31.8mV decade-1 over concentration range of 1 × 10-4-0.3 mol dm-3 urea.

Original languageEnglish
Pages (from-to)383-388
Number of pages6
JournalElectrochimica Acta
Issue number3
Publication statusPublished - 1997
Externally publishedYes


  • Biosensor
  • Electropolymerization
  • Polypyrrole
  • Urea

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Electrochemistry


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