Preparation of Bacterial Magnetite Particles for DNA Carriers

Haruko Takeyama; Satoko Kudo; Koji Sode; Moriyuki Nakamura; Tadashi Matsunaga

doi:10.1295/koron.48.319

Preparation of Bacterial Magnetite Particles for DNA Carriers

Haruko Takeyama, Satoko Kudo, Koji Sode, Moriyuki Nakamura, Tadashi Matsunaga

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We have investigated the application of bacterial magnetite particles as DNA carriers for microprojectile based gene transfer. The magnetic bacterium, Aquaspirillum sp. AMB-1, can grow aerobically to high cell densities, allowing high yields of bacterial magnetite particles to be sonicated. The optimum sonication conditions were determined and found to be dependent on culture age. Excessive sonication damaged the magnetosome membrane surrounding magnetite particles. This decreased dispersion resulted in a reduced surface area available for DNA adsorption. When compared with gold, tungsten and non membrane coated magnetite, membrane coated magnetite was a better material for DNA binding (5 μg DNA/mg magnetite). The greatest amount of DNA was bound following treatment of membrane coated magnetite with glutaraldehyde-triamine prior to incubation with DNA.

Original language	English
Pages (from-to)	319-325
Number of pages	7
Journal	KOBUNSHI RONBUNSHU
Volume	48
Issue number	5
DOIs	https://doi.org/10.1295/koron.48.319
Publication status	Published - 1991 May
Externally published	Yes

Keywords

Aquaspirillum
Bacterial Magnetite
DNA Adsorption
DNA Carrier
Magnetic Bacterium

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Materials Science (miscellaneous)
Environmental Science(all)
Polymers and Plastics

Access to Document

10.1295/koron.48.319

Cite this

@article{e8a112e817954ce3949de586ab851554,

title = "Preparation of Bacterial Magnetite Particles for DNA Carriers",

abstract = "We have investigated the application of bacterial magnetite particles as DNA carriers for microprojectile based gene transfer. The magnetic bacterium, Aquaspirillum sp. AMB-1, can grow aerobically to high cell densities, allowing high yields of bacterial magnetite particles to be sonicated. The optimum sonication conditions were determined and found to be dependent on culture age. Excessive sonication damaged the magnetosome membrane surrounding magnetite particles. This decreased dispersion resulted in a reduced surface area available for DNA adsorption. When compared with gold, tungsten and non membrane coated magnetite, membrane coated magnetite was a better material for DNA binding (5 μg DNA/mg magnetite). The greatest amount of DNA was bound following treatment of membrane coated magnetite with glutaraldehyde-triamine prior to incubation with DNA.",

keywords = "Aquaspirillum, Bacterial Magnetite, DNA Adsorption, DNA Carrier, Magnetic Bacterium",

author = "Haruko Takeyama and Satoko Kudo and Koji Sode and Moriyuki Nakamura and Tadashi Matsunaga",

year = "1991",

month = may,

doi = "10.1295/koron.48.319",

language = "English",

volume = "48",

pages = "319--325",

journal = "KOBUNSHI RONBUNSHU",

issn = "0386-2186",

publisher = "Society of Polymer Science",

number = "5",

}

TY - JOUR

T1 - Preparation of Bacterial Magnetite Particles for DNA Carriers

AU - Takeyama, Haruko

AU - Kudo, Satoko

AU - Sode, Koji

AU - Nakamura, Moriyuki

AU - Matsunaga, Tadashi

PY - 1991/5

Y1 - 1991/5

N2 - We have investigated the application of bacterial magnetite particles as DNA carriers for microprojectile based gene transfer. The magnetic bacterium, Aquaspirillum sp. AMB-1, can grow aerobically to high cell densities, allowing high yields of bacterial magnetite particles to be sonicated. The optimum sonication conditions were determined and found to be dependent on culture age. Excessive sonication damaged the magnetosome membrane surrounding magnetite particles. This decreased dispersion resulted in a reduced surface area available for DNA adsorption. When compared with gold, tungsten and non membrane coated magnetite, membrane coated magnetite was a better material for DNA binding (5 μg DNA/mg magnetite). The greatest amount of DNA was bound following treatment of membrane coated magnetite with glutaraldehyde-triamine prior to incubation with DNA.

AB - We have investigated the application of bacterial magnetite particles as DNA carriers for microprojectile based gene transfer. The magnetic bacterium, Aquaspirillum sp. AMB-1, can grow aerobically to high cell densities, allowing high yields of bacterial magnetite particles to be sonicated. The optimum sonication conditions were determined and found to be dependent on culture age. Excessive sonication damaged the magnetosome membrane surrounding magnetite particles. This decreased dispersion resulted in a reduced surface area available for DNA adsorption. When compared with gold, tungsten and non membrane coated magnetite, membrane coated magnetite was a better material for DNA binding (5 μg DNA/mg magnetite). The greatest amount of DNA was bound following treatment of membrane coated magnetite with glutaraldehyde-triamine prior to incubation with DNA.

KW - Aquaspirillum

KW - Bacterial Magnetite

KW - DNA Adsorption

KW - DNA Carrier

KW - Magnetic Bacterium

UR - http://www.scopus.com/inward/record.url?scp=0025890575&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025890575&partnerID=8YFLogxK

U2 - 10.1295/koron.48.319

DO - 10.1295/koron.48.319

M3 - Article

AN - SCOPUS:0025890575

SN - 0386-2186

VL - 48

SP - 319

EP - 325

JO - KOBUNSHI RONBUNSHU

JF - KOBUNSHI RONBUNSHU

IS - 5

ER -

Preparation of Bacterial Magnetite Particles for DNA Carriers

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this