Preparation of dehydrated powder of hemoglobin vesicles

Lin Wang; Shinji Takeoka; Eishun Tsuchida; Satoru Tokuyama; Takahiro Mashiko; Tadashi Satoh

doi:10.1002/pat.1992.220030103

Preparation of dehydrated powder of hemoglobin vesicles

Lin Wang, Shinji Takeoka, Eishun Tsuchida^*, Satoru Tokuyama, Takahiro Mashiko, Tadashi Satoh

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The carbonyl hemoglobin (CO‐Hb), which was used to prevent denaturation (metHb) during the preparation of samples, was encapsulated into lipid vesicles constituted from unsaturated phospholipid, cholesterol and unsaturated fatty acid. Unsaturated components were polymerized by γ‐irradiation to enhance the stability of bilayer membrane. An aqueous dispersion of resulting Hb vesicles was freeze‐dried in the presence of saccharides (50–200 mM) to obtain a dehydrated powder of Hb vesicles. Change in the vesicle size, the leakage of encapsulated Hb and the oxidation of Hb to metHb were not observed. Therefore, the long‐term storage of Hb vesicles can be realized as a dry powder.

Original language	English
Pages (from-to)	17-21
Number of pages	5
Journal	Polymers for Advanced Technologies
Volume	3
Issue number	1
DOIs	https://doi.org/10.1002/pat.1992.220030103
Publication status	Published - 1992 Feb

Keywords

Freeze‐drying
Hemoglobin
Polymerized lipids
Saccharides
Vesicles

ASJC Scopus subject areas

Polymers and Plastics

Access to Document

10.1002/pat.1992.220030103

Cite this

@article{45b69c5aeba14d4faaedcb69fd8dfe9d,

title = "Preparation of dehydrated powder of hemoglobin vesicles",

abstract = "The carbonyl hemoglobin (CO‐Hb), which was used to prevent denaturation (metHb) during the preparation of samples, was encapsulated into lipid vesicles constituted from unsaturated phospholipid, cholesterol and unsaturated fatty acid. Unsaturated components were polymerized by γ‐irradiation to enhance the stability of bilayer membrane. An aqueous dispersion of resulting Hb vesicles was freeze‐dried in the presence of saccharides (50–200 mM) to obtain a dehydrated powder of Hb vesicles. Change in the vesicle size, the leakage of encapsulated Hb and the oxidation of Hb to metHb were not observed. Therefore, the long‐term storage of Hb vesicles can be realized as a dry powder.",

keywords = "Freeze‐drying, Hemoglobin, Polymerized lipids, Saccharides, Vesicles",

author = "Lin Wang and Shinji Takeoka and Eishun Tsuchida and Satoru Tokuyama and Takahiro Mashiko and Tadashi Satoh",

year = "1992",

month = feb,

doi = "10.1002/pat.1992.220030103",

language = "English",

volume = "3",

pages = "17--21",

journal = "Polymers for Advanced Technologies",

issn = "1042-7147",

publisher = "John Wiley and Sons Ltd",

number = "1",

}

TY - JOUR

T1 - Preparation of dehydrated powder of hemoglobin vesicles

AU - Wang, Lin

AU - Takeoka, Shinji

AU - Tsuchida, Eishun

AU - Tokuyama, Satoru

AU - Mashiko, Takahiro

AU - Satoh, Tadashi

PY - 1992/2

Y1 - 1992/2

N2 - The carbonyl hemoglobin (CO‐Hb), which was used to prevent denaturation (metHb) during the preparation of samples, was encapsulated into lipid vesicles constituted from unsaturated phospholipid, cholesterol and unsaturated fatty acid. Unsaturated components were polymerized by γ‐irradiation to enhance the stability of bilayer membrane. An aqueous dispersion of resulting Hb vesicles was freeze‐dried in the presence of saccharides (50–200 mM) to obtain a dehydrated powder of Hb vesicles. Change in the vesicle size, the leakage of encapsulated Hb and the oxidation of Hb to metHb were not observed. Therefore, the long‐term storage of Hb vesicles can be realized as a dry powder.

AB - The carbonyl hemoglobin (CO‐Hb), which was used to prevent denaturation (metHb) during the preparation of samples, was encapsulated into lipid vesicles constituted from unsaturated phospholipid, cholesterol and unsaturated fatty acid. Unsaturated components were polymerized by γ‐irradiation to enhance the stability of bilayer membrane. An aqueous dispersion of resulting Hb vesicles was freeze‐dried in the presence of saccharides (50–200 mM) to obtain a dehydrated powder of Hb vesicles. Change in the vesicle size, the leakage of encapsulated Hb and the oxidation of Hb to metHb were not observed. Therefore, the long‐term storage of Hb vesicles can be realized as a dry powder.

KW - Freeze‐drying

KW - Hemoglobin

KW - Polymerized lipids

KW - Saccharides

KW - Vesicles

UR - http://www.scopus.com/inward/record.url?scp=84994952911&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994952911&partnerID=8YFLogxK

U2 - 10.1002/pat.1992.220030103

DO - 10.1002/pat.1992.220030103

M3 - Article

AN - SCOPUS:84994952911

SN - 1042-7147

VL - 3

SP - 17

EP - 21

JO - Polymers for Advanced Technologies

JF - Polymers for Advanced Technologies

IS - 1

ER -

Preparation of dehydrated powder of hemoglobin vesicles

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this