TY - JOUR
T1 - Production of ω3 fatty acids in marine cyanobacterium Synechococcus sp. strain NKBG 15041c via genetic engineering
AU - Yoshino, Tomoko
AU - Kakunaka, Natsumi
AU - Liang, Yue
AU - Ito, Yasuhito
AU - Maeda, Yoshiaki
AU - Nomaguchi, Tatsuhiro
AU - Matsunaga, Tadashi
AU - Tanaka, Tsuyoshi
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Omega-3 fatty acids (ω3 FAs) have attracted attention because they have various health benefits for humans. Fish oils are currently major sources of ω3 FAs, but a sustainable supply of ω3 FAs based on fish oils is problematic because of the increasing demand. In this study, the production potential of a genetically engineered marine cyanobacterium, Synechococcus sp. strain NKBG 15041c, was examined as an alternative source of ω3 FAs. A change in fatty acid composition of this cyanobacterium was successfully induced by the expression of a heterologous Δ6-desaturase, and the transformants synthesized stearidonic acid, which the wild type cannot produce. As a result of optimization of culture conditions, maximal contents of stearidonic acid and total ω3 FAs reached 12.2 ± 2.4 and 118.1 ± 3.5 mg/g, respectively. The maximal ω3 FA productivity was 4.6 ± 0.7 mg/(L⋅day). These are the highest values of the contents of stearidonic acid and ω3 FAs in genetically engineered cyanobacteria reported thus far. Therefore, genetically engineered Synechococcus sp. strain NKBG 15041c may be a promising sustainable source of ω3 fatty acids.
AB - Omega-3 fatty acids (ω3 FAs) have attracted attention because they have various health benefits for humans. Fish oils are currently major sources of ω3 FAs, but a sustainable supply of ω3 FAs based on fish oils is problematic because of the increasing demand. In this study, the production potential of a genetically engineered marine cyanobacterium, Synechococcus sp. strain NKBG 15041c, was examined as an alternative source of ω3 FAs. A change in fatty acid composition of this cyanobacterium was successfully induced by the expression of a heterologous Δ6-desaturase, and the transformants synthesized stearidonic acid, which the wild type cannot produce. As a result of optimization of culture conditions, maximal contents of stearidonic acid and total ω3 FAs reached 12.2 ± 2.4 and 118.1 ± 3.5 mg/g, respectively. The maximal ω3 FA productivity was 4.6 ± 0.7 mg/(L⋅day). These are the highest values of the contents of stearidonic acid and ω3 FAs in genetically engineered cyanobacteria reported thus far. Therefore, genetically engineered Synechococcus sp. strain NKBG 15041c may be a promising sustainable source of ω3 fatty acids.
KW - Cyanobacterium
KW - Delta-6 (Δ6) desaturase
KW - Genetic engineering
KW - Omega-3 (ω3) fatty acids
UR - http://www.scopus.com/inward/record.url?scp=85026515089&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85026515089&partnerID=8YFLogxK
U2 - 10.1007/s00253-017-8407-1
DO - 10.1007/s00253-017-8407-1
M3 - Article
C2 - 28762000
AN - SCOPUS:85026515089
SN - 0175-7598
VL - 101
SP - 6899
EP - 6905
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 18
ER -
