Production of eicosapentaenoic acid by a bacterium isolated from mackerel intestines

Masamichi Akimoto*, Tetsuya Ishii, Koji Yamagaki, Kazuhisa Ohtaguchi, Kozo Koide, Kazunaga Yazawa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)


Optimization of culture conditions for the growth rate, 5,8,11,14,17-cis-Eicosapentaenoic acid (EPA) content and EPA productivity of a bacterium isolated from Pacific mackerel intestines was investigated by use of a culture medium containing 1.00 wt% peptone and 0.50 wt% yeast extract in an artificial sea water (ASW). Cultivation temperature affected the growth rate and cellular EPA content of the bacterium. The cellular EPA content at 8°C was as great as 16.8 mg/g of dry cells, which was more than two times greater than that at 25°C (7.3 mg/g of dry cells), although the growth rate showed a maximum at 25°C. Both the yield of bacterial cells and the cellular EPA content at 25°C reached maximum values when the pH of the culture medium was nearly 7.0 and when the concentration of ASW was 100% (v/v). Under optimum culture conditions [25°C pH 7.0 and 100% (v/v) ASW], the amount of EPA accumulated in the cellular lipids reached 45.6 mg/L of culture broth after 8 hr.

Original languageEnglish
Pages (from-to)911-915
Number of pages5
JournalJournal of the American Oil Chemists' Society
Issue number12
Publication statusPublished - 1990 Dec
Externally publishedYes


  • Alteromonas putrefaciens
  • eicosapentaenoic acid
  • mackerel
  • marine bacterium

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Food Science
  • Organic Chemistry
  • Chemical Engineering(all)


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