Production of Immunoabsorbent Nanoparticles by Displaying Single-Domain Protein A on Potato Virus X

Kerstin Uhde-Holzem; Michael McBurney; Brylee David B. Tiu; Rigoberto C. Advincula; Rainer Fischer; Ulrich Commandeur; Nicole F. Steinmetz

doi:10.1002/mabi.201500280

Production of Immunoabsorbent Nanoparticles by Displaying Single-Domain Protein A on Potato Virus X

Kerstin Uhde-Holzem, Michael McBurney, Brylee David B. Tiu, Rigoberto C. Advincula, Rainer Fischer, Ulrich Commandeur^*, Nicole F. Steinmetz

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

The combination of antibodies with nanoparticles provides wide-ranging applications in biosensing. While several covalent presentation strategies have been established, there is need for alternative, non-covalent methods to provide a routine for scalable nanomanufacturing. We report the multivalent presentation of the B domain of Staphylococcus aureus protein A (SpAB) on potato virus X (PVX) nanoparticles. Three different synthetic strategies were used to obtain chimeric PVX^SpAB filaments. The protein A fragments displayed on the surface of all three PVX chimeras remained fully functional as an immunoabsorbent for antibody capture enabling biosensing. The new biomaterials presented could find applications as diagnostic tools for biomedical or environmental monitoring.

Original language	English
Pages (from-to)	231-241
Number of pages	11
Journal	Macromolecular Bioscience
Volume	16
Issue number	2
DOIs	https://doi.org/10.1002/mabi.201500280
Publication status	Published - 2016 Feb 1
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

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10.1002/mabi.201500280

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abstract = "The combination of antibodies with nanoparticles provides wide-ranging applications in biosensing. While several covalent presentation strategies have been established, there is need for alternative, non-covalent methods to provide a routine for scalable nanomanufacturing. We report the multivalent presentation of the B domain of Staphylococcus aureus protein A (SpAB) on potato virus X (PVX) nanoparticles. Three different synthetic strategies were used to obtain chimeric PVXSpAB filaments. The protein A fragments displayed on the surface of all three PVX chimeras remained fully functional as an immunoabsorbent for antibody capture enabling biosensing. The new biomaterials presented could find applications as diagnostic tools for biomedical or environmental monitoring.",

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AU - Uhde-Holzem, Kerstin

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AU - Advincula, Rigoberto C.

AU - Fischer, Rainer

AU - Commandeur, Ulrich

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AB - The combination of antibodies with nanoparticles provides wide-ranging applications in biosensing. While several covalent presentation strategies have been established, there is need for alternative, non-covalent methods to provide a routine for scalable nanomanufacturing. We report the multivalent presentation of the B domain of Staphylococcus aureus protein A (SpAB) on potato virus X (PVX) nanoparticles. Three different synthetic strategies were used to obtain chimeric PVXSpAB filaments. The protein A fragments displayed on the surface of all three PVX chimeras remained fully functional as an immunoabsorbent for antibody capture enabling biosensing. The new biomaterials presented could find applications as diagnostic tools for biomedical or environmental monitoring.

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Production of Immunoabsorbent Nanoparticles by Displaying Single-Domain Protein A on Potato Virus X

Abstract

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