We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.
- Bacterial artificial chromosome (BAC)
- Down syndrome
- Inbred mice
- Intracytoplasmic sperm injection (ICSI)
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology