Progresses in separation and sensing technologies using DNA as affinity ligands (Review)

Daisuke Umeno; Mizuo Maeda

doi:10.2116/bunsekikagaku.48.871

Progresses in separation and sensing technologies using DNA as affinity ligands (Review)

Research output: Contribution to journal › Article › peer-review

Abstract

The recent developments of affinity techniques concerning single- and double-stranded DNA are reviewed. Based on the opinion that the immobilization of DNA is essential for the analytical and preparative use of the ligand function of DNAs, the two items are extensively discussed concerning the choice of conjugation chemistry and supports. Finally, a variety of applications, mainly for sensing and purification, are discussed.

Original language	English
Pages (from-to)	871-890
Number of pages	20
Journal	BUNSEKI KAGAKU
Volume	48
Issue number	10
DOIs	https://doi.org/10.2116/bunsekikagaku.48.871
Publication status	Published - 1999
Externally published	Yes

Keywords

Affinity separation
Bioconjugate
Biosensor
DNA chip
DNA-binding protein
Hybrid material
Immobilized DNA

ASJC Scopus subject areas

Analytical Chemistry

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10.2116/bunsekikagaku.48.871

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abstract = "The recent developments of affinity techniques concerning single- and double-stranded DNA are reviewed. Based on the opinion that the immobilization of DNA is essential for the analytical and preparative use of the ligand function of DNAs, the two items are extensively discussed concerning the choice of conjugation chemistry and supports. Finally, a variety of applications, mainly for sensing and purification, are discussed.",

keywords = "Affinity separation, Bioconjugate, Biosensor, DNA chip, DNA-binding protein, Hybrid material, Immobilized DNA",

author = "Daisuke Umeno and Mizuo Maeda",

year = "1999",

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AU - Maeda, Mizuo

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AB - The recent developments of affinity techniques concerning single- and double-stranded DNA are reviewed. Based on the opinion that the immobilization of DNA is essential for the analytical and preparative use of the ligand function of DNAs, the two items are extensively discussed concerning the choice of conjugation chemistry and supports. Finally, a variety of applications, mainly for sensing and purification, are discussed.

KW - Affinity separation

KW - Bioconjugate

KW - Biosensor

KW - DNA chip

KW - DNA-binding protein

KW - Hybrid material

KW - Immobilized DNA

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Progresses in separation and sensing technologies using DNA as affinity ligands (Review)

Abstract

Keywords

ASJC Scopus subject areas

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