Prolactin augments luteinizing hormone binding to rat Leydig cells in serum-free culture

Minoru Takase*, Kazuyoshi Tsutsui, Seiichiro Kawashima

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Effect of prolactin on the testicular luteinizing hormone binding was studied in a serum-free culture system. By the collagenase digestion of decapsulated testes taken out from 25-day-old rats, Leydig cells were isolated and cultured for 7 days in DME/F12 (1:1) medium supplemented with insulin, transferrin, epidermal growth factor, and gentamicin. The cultured cells exhibited the 3β-hydroxysteroid dehydrogenase activity. Hill plots constructed from the data of competition experiment showed that the dissociation constant (Kd) was 0.33 × 10-10M. The Kd value was approximately the same as the known value for the rat testicular homogenates. When the Leydig cells were cultured with ovine prolactin for the last 3 days of 7-day culture period, the binding of luteinizing hormone increased to 1.7-fold ofthat in the control group. From these results it is concluded that prolactin acts to up-regulate the binding of luteinizing hormone to rat testicular Leydig cells in serum-free culture

Original languageEnglish
Pages (from-to)511-517
Number of pages7
JournalJournal of Biosciences
Issue number4
Publication statusPublished - 1996 Jun
Externally publishedYes


  • Leydig cell
  • luteinizing hormone binding
  • Prolactin

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology
  • General Agricultural and Biological Sciences


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