TY - JOUR
T1 - Protein functionalized micro hydrogel features for cell-surface interaction
AU - Bhatnagar, Parijat
AU - Nixon, Alan J.
AU - Kim, Il
AU - Kameoka, Jun
N1 - Funding Information:
Acknowledgements We thank Profs. Harold G. Craighead, and Barbara Baird for their guidance and support; Michael Scimeca, M. Lisa Strassheim, Ryan Young, Sueng-Min Park, Aaron Smith, and Mathieu Foquet for technical help. This material is based upon work supported in part by the Nanobiotechnology Center, an STC Program of the National Science Foundation under Agreement no. ECS-9876771. IK thanks NCRC Program from MOST/KOSEF (R15-2006-022-01001-0).
PY - 2008/8
Y1 - 2008/8
N2 - Cross-linked hydrogel features have been patterned using subtractive lift-off of polymerized hydrogel film. Projection lithography and oxygen plasma etch was used to pattern parylene C polymer film. Molecular self-assembly of polymerizable monolayer was obtained in solution-phase and acrylamide based hydrogel was polymerized using free-radical polymerization on this substrate. Parylene C film was mechanically lifted-off to remove the blanket hydrogel film and micro hydrogel features (μhf) were obtained attached to the predefined patterns in the range from 1 to 60 μm. The μhf were functionalized with aldehyde functional groups, and proteins were coupled to them using Schiff base chemistry followed by reductive amination. Interaction of mesenchymal stem cells with transforming growth factor-beta 1 (TGF-β1) functionalized μhf was studied, and TGF-β1 was found to retain its tumor suppression activity.
AB - Cross-linked hydrogel features have been patterned using subtractive lift-off of polymerized hydrogel film. Projection lithography and oxygen plasma etch was used to pattern parylene C polymer film. Molecular self-assembly of polymerizable monolayer was obtained in solution-phase and acrylamide based hydrogel was polymerized using free-radical polymerization on this substrate. Parylene C film was mechanically lifted-off to remove the blanket hydrogel film and micro hydrogel features (μhf) were obtained attached to the predefined patterns in the range from 1 to 60 μm. The μhf were functionalized with aldehyde functional groups, and proteins were coupled to them using Schiff base chemistry followed by reductive amination. Interaction of mesenchymal stem cells with transforming growth factor-beta 1 (TGF-β1) functionalized μhf was studied, and TGF-β1 was found to retain its tumor suppression activity.
KW - Biologically friendly microfabrication processes
KW - Cell-surface interaction
KW - Integrated microfabrication of hydrogels
KW - Surface immobilization of growth factors
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U2 - 10.1007/s10544-008-9167-6
DO - 10.1007/s10544-008-9167-6
M3 - Article
C2 - 18259869
AN - SCOPUS:47749102995
SN - 1387-2176
VL - 10
SP - 567
EP - 571
JO - Biomedical Microdevices
JF - Biomedical Microdevices
IS - 4
ER -