Proteomic profiling of intestinal epithelial-like cell-derived exosomes regulated by epigallocatechin gallate

Satoshi Yano*, Katsuhiko Suzuki, Taichi Hara*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Exosomes are extracellular vesicles primarily responsible for intercellular communication, and they contain nucleic acids and proteins. Exosome secretion has been observed in the intestines, suggesting their physiological effects on the receptor cells of target tissues. It is possible that intestinal epithelial cells recognize food components as ligands, resulting in exosome secretion. However, research on intestine-derived exosomes regulated by food ingredients is limited. In this study, Caco-2 cells were utilized as an intestinal epithelial model for proteomic profiling. NanoLC-MS/MS analysis revealed the alteration of exosome properties by epigallocatechin gallate (EGCG) in differentiated Caco-2 cells. This natural polyphenol reduced both the number and size of secreted exosomes and altered the expression of exosomal proteins. The enriched proteins in exosomes were involved in immune response and cell proliferation. In contrast, those in the EGCG-treated group had distinctive functions in the maintenance of skin homeostasis. We also found variable expression of galectin-3-binding protein and fibronectin as molecular signatures in exosomes derived from EGCG-treated cells. These results could help elucidate the expression and mechanism of exosomal proteins related to food components.

Original languageEnglish
Pages (from-to)390-404
Number of pages15
Issue number2
Publication statusPublished - 2023 Mar 1


  • Caco-2 cells
  • EGCG
  • exosome
  • intestinal epithelial cells
  • proteomic profiling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Clinical Biochemistry


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