Purified cell-free systems as standard parts for synthetic biology

Hideaki Matsubayashi, Takuya Ueda*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

29 Citations (Scopus)


A cell free protein synthesis approach is extensively used for biochemical and synthetic biology researches. Unlike lysate based cell free systems, the PURE system is reconstituted with individually purified factors essential for transcriptional and translational processes. Hence, the components in the PURE system can be definitely manipulated as per the desired situation. Because of this high controllability, the PURE system has been applied to a wide range of research scene, such as biochemical analysis in reconstructed system, in vitro protein engineering, reconstitution of an artificial cell. We believe that this purified cell-free protein synthesis system become a basal technology to advance synthetic biology.

Original languageEnglish
Pages (from-to)158-162
Number of pages5
JournalCurrent Opinion in Chemical Biology
Publication statusPublished - 2014 Oct 1
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry


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