Quantitative analysis of active oxygen species by potentiometry

Makoto Yuasa; Kenichi Oyaizu; Hidenori Murata; Katsuya Eguchi; Yujiro Toyoda

doi:10.1295/koron.64.90

Quantitative analysis of active oxygen species by potentiometry

Makoto Yuasa^*, Kenichi Oyaizu, Hidenori Murata, Katsuya Eguchi, Yujiro Toyoda

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) have many vital functions including sterilizing function of white blood cell, carcinostatic function, aging, cell death, inflammation, and DNA damage. Among various ROS, superoxide anion radical (O₂^-•) has been attracting great attention as a cause of human disease. We have developed an O₂^-• sensor composed of a thin film of 1-methylimidazole (Im) coordinatied Bromoiron (III) meso-tetrathienyleporphyrin ([FeBr(ttp)]) for the electro-chemical detection. In this study, highly sensitive measurements of O₂^-• by potentiometry were conducted. The potentiometric response for O₂^-• was monitored in 10 mM phosphate buffer. The O₂^-• sensor displayed a high catalytic activity for oxidation of O₂^-• and showed a linear relationship between the sensor response and the logarithm of O₂^-• concentration. This O₂^-• sensor showed low interferences due to the electrochemical interferents H₂O₂ and uric acid. Measurement of O₂ ^-• by potentiometry was able to detect the low concentrations of O₂^-• (7 × 10^-7 M) that measurements of O₂^-• by amperometry could not easily detect.

Original language	English
Pages (from-to)	90-95
Number of pages	6
Journal	KOBUNSHI RONBUNSHU
Volume	64
Issue number	2
DOIs	https://doi.org/10.1295/koron.64.90
Publication status	Published - 2007 Feb
Externally published	Yes

Keywords

Electrochemical sensor
Ironporphyrin
Potentiometry
Superoxide anion radical

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Materials Science (miscellaneous)
Environmental Science(all)
Polymers and Plastics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1295/koron.64.90

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title = "Quantitative analysis of active oxygen species by potentiometry",

abstract = "Reactive oxygen species (ROS) have many vital functions including sterilizing function of white blood cell, carcinostatic function, aging, cell death, inflammation, and DNA damage. Among various ROS, superoxide anion radical (O2-•) has been attracting great attention as a cause of human disease. We have developed an O2-• sensor composed of a thin film of 1-methylimidazole (Im) coordinatied Bromoiron (III) meso-tetrathienyleporphyrin ([FeBr(ttp)]) for the electro-chemical detection. In this study, highly sensitive measurements of O2-• by potentiometry were conducted. The potentiometric response for O2-• was monitored in 10 mM phosphate buffer. The O2-• sensor displayed a high catalytic activity for oxidation of O2-• and showed a linear relationship between the sensor response and the logarithm of O2-• concentration. This O2-• sensor showed low interferences due to the electrochemical interferents H2O2 and uric acid. Measurement of O2 -• by potentiometry was able to detect the low concentrations of O2-• (7 × 10-7 M) that measurements of O2-• by amperometry could not easily detect.",

keywords = "Electrochemical sensor, Ironporphyrin, Potentiometry, Superoxide anion radical",

author = "Makoto Yuasa and Kenichi Oyaizu and Hidenori Murata and Katsuya Eguchi and Yujiro Toyoda",

year = "2007",

month = feb,

doi = "10.1295/koron.64.90",

language = "English",

volume = "64",

pages = "90--95",

journal = "KOBUNSHI RONBUNSHU",

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TY - JOUR

T1 - Quantitative analysis of active oxygen species by potentiometry

AU - Yuasa, Makoto

AU - Oyaizu, Kenichi

AU - Murata, Hidenori

AU - Eguchi, Katsuya

AU - Toyoda, Yujiro

PY - 2007/2

Y1 - 2007/2

N2 - Reactive oxygen species (ROS) have many vital functions including sterilizing function of white blood cell, carcinostatic function, aging, cell death, inflammation, and DNA damage. Among various ROS, superoxide anion radical (O2-•) has been attracting great attention as a cause of human disease. We have developed an O2-• sensor composed of a thin film of 1-methylimidazole (Im) coordinatied Bromoiron (III) meso-tetrathienyleporphyrin ([FeBr(ttp)]) for the electro-chemical detection. In this study, highly sensitive measurements of O2-• by potentiometry were conducted. The potentiometric response for O2-• was monitored in 10 mM phosphate buffer. The O2-• sensor displayed a high catalytic activity for oxidation of O2-• and showed a linear relationship between the sensor response and the logarithm of O2-• concentration. This O2-• sensor showed low interferences due to the electrochemical interferents H2O2 and uric acid. Measurement of O2 -• by potentiometry was able to detect the low concentrations of O2-• (7 × 10-7 M) that measurements of O2-• by amperometry could not easily detect.

AB - Reactive oxygen species (ROS) have many vital functions including sterilizing function of white blood cell, carcinostatic function, aging, cell death, inflammation, and DNA damage. Among various ROS, superoxide anion radical (O2-•) has been attracting great attention as a cause of human disease. We have developed an O2-• sensor composed of a thin film of 1-methylimidazole (Im) coordinatied Bromoiron (III) meso-tetrathienyleporphyrin ([FeBr(ttp)]) for the electro-chemical detection. In this study, highly sensitive measurements of O2-• by potentiometry were conducted. The potentiometric response for O2-• was monitored in 10 mM phosphate buffer. The O2-• sensor displayed a high catalytic activity for oxidation of O2-• and showed a linear relationship between the sensor response and the logarithm of O2-• concentration. This O2-• sensor showed low interferences due to the electrochemical interferents H2O2 and uric acid. Measurement of O2 -• by potentiometry was able to detect the low concentrations of O2-• (7 × 10-7 M) that measurements of O2-• by amperometry could not easily detect.

KW - Electrochemical sensor

KW - Ironporphyrin

KW - Potentiometry

KW - Superoxide anion radical

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Quantitative analysis of active oxygen species by potentiometry

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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