Abstract
Tissue vascularization is one of the biggest challenges in tissue engineering. We constructed 3D hepatocyte tissue-like structures and endothelial capillary networks in a microfluidic platform. Here, we analyzed interactions between these structures quantitatively by recording the cell migration and morphogenesis. The analysis of capillary network extension rate under various combinations of growth factors (e.g., HGF, VEGF) revealed that the growth rate significantly increased by the growth factor addition. The capillary networks, however, stopped extending after contacting with hepatocytes, suggesting that additional factors are necessary for achieving hepatocyte tissue-like structures including microvascular networks.
| Original language | English |
|---|---|
| Title of host publication | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
| Pages | 1529-1531 |
| Number of pages | 3 |
| Volume | 3 |
| Publication status | Published - 2011 |
| Externally published | Yes |
| Event | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA Duration: 2011 Oct 2 → 2011 Oct 6 |
Other
| Other | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
|---|---|
| City | Seattle, WA |
| Period | 11/10/2 → 11/10/6 |
Keywords
- Hepatocyte
- Tissue Engineering
- Vascularization
ASJC Scopus subject areas
- Control and Systems Engineering