Radial glial cell-specific ablation in the adult Zebrafish brain

Yuki Shimizu, Yoko Ito, Hideomi Tanaka, Toshio Ohshima*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The zebrafish brain can continue to produce new neurons in widespread neurogenic brain regions throughout life. In contrast, neurogenesis in the adult mammalian brain is restricted to the subventricular zone (SVZ) and dentate gyrus (DG). In neurogenic regions in the adult brain, radial glial cells (RGCs) are considered to function as neural stem cells (NSCs). We generated a Tg(gfap:Gal4FF) transgenic zebrafish line, which enabled us to express specific genes in RGCs. To study the function of RGCs in neurogenesis in the adult zebrafish brain, we also generated a Tg(gfap: Gal4FF; UAS:nfsB-mcherry) transgenic zebrafish line, which allowed us to induce cell death exclusively within RGCs upon addition of metronidazole (Mtz) to the media. RGCs expressing nitroreductase were specifically ablated by the Mtz treatment, decreasing the number of proliferative RGCs. Using the Tg(gfap:Gal4FF; UAS:nfsB-mcherry) transgenic zebrafish line, we found that RGCs were specifically ablated in the adult zebrafish telencephalon. The Tg(gfap:Gal4FF) line could be useful to study the function of RGCs.

Original languageEnglish
Pages (from-to)431-439
Number of pages9
JournalGenesis
Volume53
Issue number7
DOIs
Publication statusPublished - 2015 Jul 1

Keywords

  • Adult neurogenesis
  • Cell ablation
  • Gal4-UAS
  • Transgenic
  • Zebrafish

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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