TY - JOUR
T1 - Radiation-induced graft polymerization is the key to develop high- performance functional materials for protein purification
AU - Saito, Kyoichi
AU - Tsuneda, Satoshi
AU - Kim, Min
AU - Kubota, Noboru
AU - Sugita, Kazuyuki
AU - Sugo, Takanobu
N1 - Funding Information:
We thank William Lee, Tsuyoshi Yoshida, and Jun-ichi Kanno for preparing the schematic illustrations. This work was supported by a Toray Science and Technology Grant.
PY - 1999/5
Y1 - 1999/5
N2 - We have described a preparation scheme for immobilizing polymer chains at a uniformly high density onto a microfiltration membrane. Highly efficient protein recovery was demonstrated by the results of the determination of breakthrough and elution curves. The three requirements of high rate, high capacity, and repeated use for the protein recovery were satisfied by ensuring the occurrence of convection, multilayer binding, and hydrophilization, respectively. In addition, easy scale-up to fabrication of a membrane module was verified on a small scale.
AB - We have described a preparation scheme for immobilizing polymer chains at a uniformly high density onto a microfiltration membrane. Highly efficient protein recovery was demonstrated by the results of the determination of breakthrough and elution curves. The three requirements of high rate, high capacity, and repeated use for the protein recovery were satisfied by ensuring the occurrence of convection, multilayer binding, and hydrophilization, respectively. In addition, easy scale-up to fabrication of a membrane module was verified on a small scale.
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U2 - 10.1016/S0969-806X(98)00256-4
DO - 10.1016/S0969-806X(98)00256-4
M3 - Article
AN - SCOPUS:0033137044
SN - 0969-806X
VL - 54
SP - 517
EP - 525
JO - Radiation Physics and Chemistry
JF - Radiation Physics and Chemistry
IS - 5
ER -