TY - JOUR
T1 - Rapid and selective discrimination of gram-positive and gram-negative bacteria by boronic acid-modified poly(amidoamine) dendrimer
AU - Tsuchido, Yuji
AU - Horiuchi, Ryosuke
AU - Hashimoto, Takeshi
AU - Ishihara, Kanako
AU - Kanzawa, Nobuyuki
AU - Hayashita, Takashi
N1 - Funding Information:
This work was financially supported by a Grant-in-Aid for Early-Career Scientists (Grant No. 18K14255) and a Grant-in-Aid for Scientific Research (C) (Grant No. 18K05180) from Japan Society for the Promotion of Science (JSPS) and a Grant-in Aid for Scientific Research (A) (Grant No. 26248038) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan.
Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/3/19
Y1 - 2019/3/19
N2 - There is an urgent need to develop a rapid and selective method for the detection of bacteria because delayed diagnosis and the overuse of antibiotics have triggered drug resistance in bacteria. To this end, we prepared boronic acid-modified poly(amidoamine) generation 4 (B-PAMAM(G4)) dendrimer as cross-linking molecules that form aggregates with bacteria. Within 5 min of adding B-PAMAM(G4) dendrimer solution to a bacterial suspension, large aggregates were observed. Interestingly, the aggregate formation with various bacteria was pH-dependent. In basic pH, both Gram-positive and Gram-negative bacteria formed aggregates, but in neutral pH, only Gram-positive bacteria formed aggregates. We revealed that this bacteria-selective aggregation involved the bacterial surface recognition of the phenylboronic acid moiety of B-PAMAM(G4) dendrimer. In addition, we demonstrated that the spherical structure of B-PAMAM(G4) was one of the important factors for the formation of large aggregates. The aggregation was also observed in the presence of ≤10 mM fructose. B-PAMAM(G4) dendrimer is expected to be a powerful tool for the rapid and selective discrimination between Gram-positive and Gram-negative bacteria.
AB - There is an urgent need to develop a rapid and selective method for the detection of bacteria because delayed diagnosis and the overuse of antibiotics have triggered drug resistance in bacteria. To this end, we prepared boronic acid-modified poly(amidoamine) generation 4 (B-PAMAM(G4)) dendrimer as cross-linking molecules that form aggregates with bacteria. Within 5 min of adding B-PAMAM(G4) dendrimer solution to a bacterial suspension, large aggregates were observed. Interestingly, the aggregate formation with various bacteria was pH-dependent. In basic pH, both Gram-positive and Gram-negative bacteria formed aggregates, but in neutral pH, only Gram-positive bacteria formed aggregates. We revealed that this bacteria-selective aggregation involved the bacterial surface recognition of the phenylboronic acid moiety of B-PAMAM(G4) dendrimer. In addition, we demonstrated that the spherical structure of B-PAMAM(G4) was one of the important factors for the formation of large aggregates. The aggregation was also observed in the presence of ≤10 mM fructose. B-PAMAM(G4) dendrimer is expected to be a powerful tool for the rapid and selective discrimination between Gram-positive and Gram-negative bacteria.
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U2 - 10.1021/acs.analchem.8b04870
DO - 10.1021/acs.analchem.8b04870
M3 - Article
C2 - 30652471
AN - SCOPUS:85061251851
SN - 0003-2700
VL - 91
SP - 3929
EP - 3935
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 6
ER -