TY - JOUR
T1 - Rapid and sensitive detection of 17β-estradiol in environmental water using automated immunoassay system with bacterial magnetic particles
AU - Tanaka, Tsuyoshi
AU - Takeda, Hajime
AU - Ueki, Fumiko
AU - Obata, Kimimichi
AU - Tajima, Hideji
AU - Takeyama, Haruko
AU - Goda, Yasuhiro
AU - Fujimoto, Shigeru
AU - Matsunaga, Tadashi
N1 - Funding Information:
This work was funded in part by a Grant-in-Aid for Specially Promoted Research (2), No. 13002005 and Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan. It was also supported by the Proposal-Based New Industrial Creative Type Technology R&D Program from the New Energy and Industrial Technology Development Organization (NEDO) of Japan.
PY - 2004/3/4
Y1 - 2004/3/4
N2 - A fully automated immunoassay of 17β-estradiol (E2) was performed using anti-E2 monoclonal antibody immobilized on bacterial magnetic particles (AntiE2-BMPs) and alkaline phosphatase-conjugated E2 (ALP-E2). E2 concentration in environmental water samples was evaluated by decrease in luminescence based on competitive reaction. A linear correlation between the luminescence intensity and E2 concentration was obtained between 0.5 and 5ppb. The minimum detectable concentration of E2 was 20ppt. All measurement steps were done within 0.5h. The analysis of environmental water samples by a commercially available ELISA kit and the BMP-based immunoassay gave good correlation plots with a correlation efficient of 0.992. These results suggest that the fully automated system using the BMP-based immunoassay has some advantages in the high rapidity and sensitivity of the measurement. This system will enable us to determine low E2 concentrations without sample condensation.
AB - A fully automated immunoassay of 17β-estradiol (E2) was performed using anti-E2 monoclonal antibody immobilized on bacterial magnetic particles (AntiE2-BMPs) and alkaline phosphatase-conjugated E2 (ALP-E2). E2 concentration in environmental water samples was evaluated by decrease in luminescence based on competitive reaction. A linear correlation between the luminescence intensity and E2 concentration was obtained between 0.5 and 5ppb. The minimum detectable concentration of E2 was 20ppt. All measurement steps were done within 0.5h. The analysis of environmental water samples by a commercially available ELISA kit and the BMP-based immunoassay gave good correlation plots with a correlation efficient of 0.992. These results suggest that the fully automated system using the BMP-based immunoassay has some advantages in the high rapidity and sensitivity of the measurement. This system will enable us to determine low E2 concentrations without sample condensation.
KW - 17β-Estradiol (E2)
KW - Anti-E2 monoclonal antibody
KW - Bacterial magnetic particles (BMPs)
KW - Fully automated immunoassay system
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U2 - 10.1016/j.jbiotec.2003.11.010
DO - 10.1016/j.jbiotec.2003.11.010
M3 - Article
C2 - 15129724
AN - SCOPUS:0842281273
SN - 0168-1656
VL - 108
SP - 153
EP - 159
JO - Journal of Biotechnology
JF - Journal of Biotechnology
IS - 2
ER -