TY - JOUR
T1 - Rapid detection of the neonicotinoid insecticide imidacloprid using a quenchbody assay
AU - Zhao, Shitao
AU - Dong, Jinhua
AU - Jeong, Hee Jin
AU - Okumura, Koichi
AU - Ueda, Hiroshi
N1 - Funding Information:
Funding information The authors are partly supported by JSPS KAKENHI (Grant Nos. JP15H04191, JP18H03851 and JP26420793) from the Japan Society for the Promotion of Science; by a Special Developing Research Grant from the Nakatani Foundation, Japan; and by Natural Science Foundation of Shandong Province (Grant Number: ZR2017MB037) and National Natural Science Foundation of China (Grant Number: 21775064).
Publisher Copyright:
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Contamination of the land and water by neonicotinoid insecticide residues is currently a severe environmental problem. However, the traditional methods for pesticide residue analysis are time consuming and laborious. To tackle this problem, here we describe a novel quenchbody (Q-body) immunoassay reagent that allows the rapid and sensitive detection of imidacloprid, one of the most frequently used neonicotinoid pesticides, in aqueous solution. A Q-body comprises an antibody Fab fragment that is site-specifically labeled with a fluorescent dye. The Fab fragment quenches the dye with its internal tryptophan residues via photoinduced electron transfer. The subsequent addition of imidacloprid stabilizes the antibody structure and displaces the quenched dye to the outside of the protein, resulting in increased fluorescence. The constructed Q-body assay exhibited a high dynamic range and a low limit of detection (10 ng mL−1), and the entire assay procedure could be completed in a few minutes. The assay showed a low cross-reactivity with possible interfering analogous compounds, indicating that it has a good selectivity. Hence, the developed Q-body assay has excellent potential as a universal technology for monitoring neonicotinoid residues in environmental and food samples. [Figure not available: see fulltext.].
AB - Contamination of the land and water by neonicotinoid insecticide residues is currently a severe environmental problem. However, the traditional methods for pesticide residue analysis are time consuming and laborious. To tackle this problem, here we describe a novel quenchbody (Q-body) immunoassay reagent that allows the rapid and sensitive detection of imidacloprid, one of the most frequently used neonicotinoid pesticides, in aqueous solution. A Q-body comprises an antibody Fab fragment that is site-specifically labeled with a fluorescent dye. The Fab fragment quenches the dye with its internal tryptophan residues via photoinduced electron transfer. The subsequent addition of imidacloprid stabilizes the antibody structure and displaces the quenched dye to the outside of the protein, resulting in increased fluorescence. The constructed Q-body assay exhibited a high dynamic range and a low limit of detection (10 ng mL−1), and the entire assay procedure could be completed in a few minutes. The assay showed a low cross-reactivity with possible interfering analogous compounds, indicating that it has a good selectivity. Hence, the developed Q-body assay has excellent potential as a universal technology for monitoring neonicotinoid residues in environmental and food samples. [Figure not available: see fulltext.].
KW - Biosensors
KW - Colony collapsing disorder
KW - Immunoassay
KW - Neonicotinoid
KW - On-site monitoring
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U2 - 10.1007/s00216-018-1074-y
DO - 10.1007/s00216-018-1074-y
M3 - Article
C2 - 29704031
AN - SCOPUS:85046010811
SN - 1618-2642
VL - 410
SP - 4219
EP - 4226
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 17
ER -