Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen

Tatsuya Munaka; Hirohisa Abe; Masaki Kanai; Takashi Sakamoto; Hiroaki Nakanishi; Shuichi Shoji; Shinya Kimura; Taira Maekawa; Akira Murakami

doi:10.1039/b701049d

Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen

Tatsuya Munaka, Hirohisa Abe, Masaki Kanai, Takashi Sakamoto, Hiroaki Nakanishi, Shuichi Shoji, Shinya Kimura, Taira Maekawa, Akira Murakami^*

^*Corresponding author for this work

School of Fundamental Science and Engineering

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

This paper describes the cultivation of primary cells in a microchamber and the real-time monitoring of small amounts of antibody secretion after the introduction of a minute amount of stimulus by a microinjector using time-resolved fluorescence anisotropy analysis.

Original language	English
Pages (from-to)	512-514
Number of pages	3
Journal	Analyst
Volume	132
Issue number	6
DOIs	https://doi.org/10.1039/b701049d
Publication status	Published - 2007 Jan 1

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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10.1039/b701049d

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title = "Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen",

abstract = "This paper describes the cultivation of primary cells in a microchamber and the real-time monitoring of small amounts of antibody secretion after the introduction of a minute amount of stimulus by a microinjector using time-resolved fluorescence anisotropy analysis.",

author = "Tatsuya Munaka and Hirohisa Abe and Masaki Kanai and Takashi Sakamoto and Hiroaki Nakanishi and Shuichi Shoji and Shinya Kimura and Taira Maekawa and Akira Murakami",

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AU - Munaka, Tatsuya

AU - Abe, Hirohisa

AU - Kanai, Masaki

AU - Sakamoto, Takashi

AU - Nakanishi, Hiroaki

AU - Shoji, Shuichi

AU - Kimura, Shinya

AU - Maekawa, Taira

AU - Murakami, Akira

PY - 2007/1/1

Y1 - 2007/1/1

N2 - This paper describes the cultivation of primary cells in a microchamber and the real-time monitoring of small amounts of antibody secretion after the introduction of a minute amount of stimulus by a microinjector using time-resolved fluorescence anisotropy analysis.

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Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen

Abstract

ASJC Scopus subject areas

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