Reconstitution of peptide bond formation with Escherichia coli 23S ribosomal RNA domains

Itaru Nitta; Yoshie Kamada; Hiroe Noda; Takuya Ueda; Kimitsuna Watanabe

doi:10.1126/science.281.5377.666

Reconstitution of peptide bond formation with Escherichia coli 23S ribosomal RNA domains

Itaru Nitta, Yoshie Kamada, Hiroe Noda, Takuya Ueda, Kimitsuna Watanabe^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

61 Citations (Scopus)

Abstract

It was recently demonstrated that peptide bond formation can occur using an Escherichia coli naked 23S ribosomal RNA without any of the ribosomal proteins. Here, the six domains of the 23S ribosomal RNA were individually synthesized and shown to be capable, when complexed together, of stimulating the reaction. Omission and addition experiments indicated that the activity could be reconstituted solely by domain V at a concentration 10 times higher than that of the intact 23S ribosomal RNA, whereas domain VI could enhance the activity in trans. These findings suggest that fragments of an RNA molecule have the ability to associate into a functional whole.

Original language	English
Pages (from-to)	666-669
Number of pages	4
Journal	Science
Volume	281
Issue number	5377
DOIs	https://doi.org/10.1126/science.281.5377.666
Publication status	Published - 1998 Jul 31
Externally published	Yes

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AU - Nitta, Itaru

AU - Kamada, Yoshie

AU - Noda, Hiroe

AU - Ueda, Takuya

AU - Watanabe, Kimitsuna

PY - 1998/7/31

Y1 - 1998/7/31

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AB - It was recently demonstrated that peptide bond formation can occur using an Escherichia coli naked 23S ribosomal RNA without any of the ribosomal proteins. Here, the six domains of the 23S ribosomal RNA were individually synthesized and shown to be capable, when complexed together, of stimulating the reaction. Omission and addition experiments indicated that the activity could be reconstituted solely by domain V at a concentration 10 times higher than that of the intact 23S ribosomal RNA, whereas domain VI could enhance the activity in trans. These findings suggest that fragments of an RNA molecule have the ability to associate into a functional whole.

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Reconstitution of peptide bond formation with Escherichia coli 23S ribosomal RNA domains

Abstract

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