TY - JOUR
T1 - Reconstruction of the Eukaryotic Communities in Beppu Bay Over the Past 50 Years Based on Sedimentary DNA Barcoding
AU - Segawa, Yudai
AU - Yamamoto, Masanobu
AU - Kuwae, Michinobu
AU - Moriya, Kazuyoshi
AU - Suzuki, Hitoshi
AU - Suzuki, Koji
N1 - Funding Information:
The authors thank Hidejiro Onishi for core sampling using R/V ISANA, Ehime University. The authors acknowledge financial support by the Japan Society for the Promotion of Science to MY (JPMXS05R2900001 and 19H0559519H05595) and to MK (18H01292). The cooperative research program (18A024 and 19A007) of the Center for Advanced Marine Core Research, Kochi University, also supported this study. Comments by two anonymous reviewers improved this manuscript.
Funding Information:
The authors thank Hidejiro Onishi for core sampling using R/V ISANA, Ehime University. The authors acknowledge financial support by the Japan Society for the Promotion of Science to MY (JPMXS05R2900001 and 19H0559519H05595) and to MK (18H01292). The cooperative research program (18A024 and 19A007) of the Center for Advanced Marine Core Research, Kochi University, also supported this study. Comments by two anonymous reviewers improved this manuscript.
Publisher Copyright:
© 2022 American Geophysical Union. All Rights Reserved.
PY - 2022/6
Y1 - 2022/6
N2 - Comprehensive reconstruction of changes in eukaryotic communities in the recent past is useful for determining the response of the local ecosystems to global changes during the Anthropocene. We used DNA barcoding technology to reconstruct the marine eukaryotic communities of Beppu Bay, the Seto Inland Sea, Japan, over the past 50 years based on a short sediment core. Highly vulnerable DNA fragments were preserved in the sediments, possibly due to seasonally euxinic conditions. Analysis of the 18S rRNA V9 gene region indicated the temporal variability in eukaryotic communities, which consisted mainly of dinoflagellates and diatoms, in response to changes in the nutrient regime. The dominant species in the dinoflagellate genus Alexandrium changed as the water temperature increased. In addition, enhanced contributions by terrestrial plants and mosses were detected in flood sediments. Our results suggest that DNA fragments can be used as a proxy for the paleoenvironmental and paleoecological conditions in Beppu Bay.
AB - Comprehensive reconstruction of changes in eukaryotic communities in the recent past is useful for determining the response of the local ecosystems to global changes during the Anthropocene. We used DNA barcoding technology to reconstruct the marine eukaryotic communities of Beppu Bay, the Seto Inland Sea, Japan, over the past 50 years based on a short sediment core. Highly vulnerable DNA fragments were preserved in the sediments, possibly due to seasonally euxinic conditions. Analysis of the 18S rRNA V9 gene region indicated the temporal variability in eukaryotic communities, which consisted mainly of dinoflagellates and diatoms, in response to changes in the nutrient regime. The dominant species in the dinoflagellate genus Alexandrium changed as the water temperature increased. In addition, enhanced contributions by terrestrial plants and mosses were detected in flood sediments. Our results suggest that DNA fragments can be used as a proxy for the paleoenvironmental and paleoecological conditions in Beppu Bay.
KW - Beppu Bay
KW - DNA barcoding
KW - eukaryotic communities
KW - nutrient availability
KW - sedaDNA
KW - the Anthropocene
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U2 - 10.1029/2022JG006825
DO - 10.1029/2022JG006825
M3 - Article
AN - SCOPUS:85132865225
SN - 2169-8953
VL - 127
JO - Journal of Geophysical Research G: Biogeosciences
JF - Journal of Geophysical Research G: Biogeosciences
IS - 6
M1 - e2022JG006825
ER -