Recovery of a marine cyanobacterial recombinant product using fish-feed organisms

Koji Sode; Toshihiro Hayashi; Masahiro Tatara; Naoaki Hatano; Hiromi Yoshida; Haruko Takeyama; Takashi Oshiro; Tadashi Matsunaga

Recovery of a marine cyanobacterial recombinant product using fish-feed organisms

Koji Sode^*, Toshihiro Hayashi, Masahiro Tatara, Naoaki Hatano, Hiromi Yoshida, Haruko Takeyama, Takashi Oshiro, Tadashi Matsunaga

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A novel approach for the recovery of recombinant products expressed in marine cyanobacteria, utilizing the fish-feed organism, brine shrimp (Artemia salina), is presented. Chloramphenicol acetyltransferase (CAT) was highly expressed in the marine unicellular cyanobacterium, Synechococcus sp. NKBG15041c, under a c-phycocyanin promoter derived from Synechococcus sp. PCC7942. These recombinant marine cyanobacteria were recovered using brine shrimp. CAT activity was retained in brine shrimp and increased with the time they were fed with recombinant cyanobacteria expressing CAT. Subsequently, the brine shrimp were fed to medaka fish. About 80% of added CAT was observed in the digestive organs of medaka for more than 9 h after feeding. These results demonstrate a novel process that can deliver a recombinant product to fish via fish-feed organisms without any purification steps.

Original language	English
Pages (from-to)	82-86
Number of pages	5
Journal	Journal of Marine Biotechnology
Volume	4
Issue number	2
Publication status	Published - 1996 Dec 1
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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abstract = "A novel approach for the recovery of recombinant products expressed in marine cyanobacteria, utilizing the fish-feed organism, brine shrimp (Artemia salina), is presented. Chloramphenicol acetyltransferase (CAT) was highly expressed in the marine unicellular cyanobacterium, Synechococcus sp. NKBG15041c, under a c-phycocyanin promoter derived from Synechococcus sp. PCC7942. These recombinant marine cyanobacteria were recovered using brine shrimp. CAT activity was retained in brine shrimp and increased with the time they were fed with recombinant cyanobacteria expressing CAT. Subsequently, the brine shrimp were fed to medaka fish. About 80% of added CAT was observed in the digestive organs of medaka for more than 9 h after feeding. These results demonstrate a novel process that can deliver a recombinant product to fish via fish-feed organisms without any purification steps.",

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T1 - Recovery of a marine cyanobacterial recombinant product using fish-feed organisms

AU - Sode, Koji

AU - Hayashi, Toshihiro

AU - Tatara, Masahiro

AU - Hatano, Naoaki

AU - Yoshida, Hiromi

AU - Takeyama, Haruko

AU - Oshiro, Takashi

AU - Matsunaga, Tadashi

PY - 1996/12/1

Y1 - 1996/12/1

N2 - A novel approach for the recovery of recombinant products expressed in marine cyanobacteria, utilizing the fish-feed organism, brine shrimp (Artemia salina), is presented. Chloramphenicol acetyltransferase (CAT) was highly expressed in the marine unicellular cyanobacterium, Synechococcus sp. NKBG15041c, under a c-phycocyanin promoter derived from Synechococcus sp. PCC7942. These recombinant marine cyanobacteria were recovered using brine shrimp. CAT activity was retained in brine shrimp and increased with the time they were fed with recombinant cyanobacteria expressing CAT. Subsequently, the brine shrimp were fed to medaka fish. About 80% of added CAT was observed in the digestive organs of medaka for more than 9 h after feeding. These results demonstrate a novel process that can deliver a recombinant product to fish via fish-feed organisms without any purification steps.

AB - A novel approach for the recovery of recombinant products expressed in marine cyanobacteria, utilizing the fish-feed organism, brine shrimp (Artemia salina), is presented. Chloramphenicol acetyltransferase (CAT) was highly expressed in the marine unicellular cyanobacterium, Synechococcus sp. NKBG15041c, under a c-phycocyanin promoter derived from Synechococcus sp. PCC7942. These recombinant marine cyanobacteria were recovered using brine shrimp. CAT activity was retained in brine shrimp and increased with the time they were fed with recombinant cyanobacteria expressing CAT. Subsequently, the brine shrimp were fed to medaka fish. About 80% of added CAT was observed in the digestive organs of medaka for more than 9 h after feeding. These results demonstrate a novel process that can deliver a recombinant product to fish via fish-feed organisms without any purification steps.

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Recovery of a marine cyanobacterial recombinant product using fish-feed organisms

Abstract

ASJC Scopus subject areas

UN SDGs

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