Abstract
Submega-sized regions of the Bacillus subtilis genome were cloned to plasmid by the B. subtilis Recombinational Transfer (BReT) method. BReT efficiency depends not only on the genome location but also on the choice of sequences for simultaneous homologous recombination during BReT. In an extreme case, a 91-kb region that was unsuccessful on the first attempt was obtained when the slightly shifted 98-kb region was targeted.
| Original language | English |
|---|---|
| Pages (from-to) | 1382-1384 |
| Number of pages | 3 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 68 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2004 Jun |
| Externally published | Yes |
Keywords
- Giant plasmid
- Homologous recombination
- Landing pad sequence
- Recombinational transfer
ASJC Scopus subject areas
- Food Science
- Applied Microbiology and Biotechnology
- Chemistry (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology