Relationship between macrophage differentiation and the chemotactic activity toward damaged myoblast cells

Masataka Uchida; Eri Oyanagi; Motohiko Miyachi; Akira Yamauchi; Hiromi Yano

doi:10.1016/j.jim.2013.04.007

Relationship between macrophage differentiation and the chemotactic activity toward damaged myoblast cells

Masataka Uchida, Eri Oyanagi, Motohiko Miyachi, Akira Yamauchi, Hiromi Yano^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We investigated the effect of macrophage differentiation on the chemotactic activity to invade local damaged myoblasts using in vitro models of muscle injury. We found that: 1) the chemotactic activity of macrophages toward areas of damaged myoblasts might be induced more by live myoblasts than dead ones, 2) the chemotactic activity of macrophages is not due to velocity, but depends on the directionality toward damaged myoblast cells, and 3) macrophage differentiation strongly influence the chemotactic activity toward damaged myoblast cells through the expression of CCR2 and/or F-actin.

Original language	English
Pages (from-to)	61-69
Number of pages	9
Journal	Journal of Immunological Methods
Volume	393
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jim.2013.04.007
Publication status	Published - 2013
Externally published	Yes

Keywords

CCR2
Dexamethasone
F-actin
Lipopolysaccharide
TAXIScan

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/j.jim.2013.04.007

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title = "Relationship between macrophage differentiation and the chemotactic activity toward damaged myoblast cells",

abstract = "We investigated the effect of macrophage differentiation on the chemotactic activity to invade local damaged myoblasts using in vitro models of muscle injury. We found that: 1) the chemotactic activity of macrophages toward areas of damaged myoblasts might be induced more by live myoblasts than dead ones, 2) the chemotactic activity of macrophages is not due to velocity, but depends on the directionality toward damaged myoblast cells, and 3) macrophage differentiation strongly influence the chemotactic activity toward damaged myoblast cells through the expression of CCR2 and/or F-actin.",

keywords = "CCR2, Dexamethasone, F-actin, Lipopolysaccharide, TAXIScan",

author = "Masataka Uchida and Eri Oyanagi and Motohiko Miyachi and Akira Yamauchi and Hiromi Yano",

note = "Funding Information: This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan ( A-23240089 and C-24500878 ) and the Interdepartmental Research Fund of Kawasaki University of Medical Welfare (to H.Y.). We thank the staff of the Environment Research Medical Center, the Tissue & Electromagnetic Microscopy Center, and the Tissue Culture and Immunity Center of Kawasaki Medical School.",

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doi = "10.1016/j.jim.2013.04.007",

language = "English",

volume = "393",

pages = "61--69",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

number = "1-2",

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T1 - Relationship between macrophage differentiation and the chemotactic activity toward damaged myoblast cells

AU - Uchida, Masataka

AU - Oyanagi, Eri

AU - Miyachi, Motohiko

AU - Yamauchi, Akira

AU - Yano, Hiromi

N1 - Funding Information: This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan ( A-23240089 and C-24500878 ) and the Interdepartmental Research Fund of Kawasaki University of Medical Welfare (to H.Y.). We thank the staff of the Environment Research Medical Center, the Tissue & Electromagnetic Microscopy Center, and the Tissue Culture and Immunity Center of Kawasaki Medical School.

PY - 2013

Y1 - 2013

N2 - We investigated the effect of macrophage differentiation on the chemotactic activity to invade local damaged myoblasts using in vitro models of muscle injury. We found that: 1) the chemotactic activity of macrophages toward areas of damaged myoblasts might be induced more by live myoblasts than dead ones, 2) the chemotactic activity of macrophages is not due to velocity, but depends on the directionality toward damaged myoblast cells, and 3) macrophage differentiation strongly influence the chemotactic activity toward damaged myoblast cells through the expression of CCR2 and/or F-actin.

AB - We investigated the effect of macrophage differentiation on the chemotactic activity to invade local damaged myoblasts using in vitro models of muscle injury. We found that: 1) the chemotactic activity of macrophages toward areas of damaged myoblasts might be induced more by live myoblasts than dead ones, 2) the chemotactic activity of macrophages is not due to velocity, but depends on the directionality toward damaged myoblast cells, and 3) macrophage differentiation strongly influence the chemotactic activity toward damaged myoblast cells through the expression of CCR2 and/or F-actin.

KW - CCR2

KW - Dexamethasone

KW - F-actin

KW - Lipopolysaccharide

KW - TAXIScan

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DO - 10.1016/j.jim.2013.04.007

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JO - Journal of Immunological Methods

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Relationship between macrophage differentiation and the chemotactic activity toward damaged myoblast cells

Abstract

Keywords

ASJC Scopus subject areas

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