Requirement of phospholipase Cδ4 for the zona pellucida-induced acrosome reaction

K. Fukami; K. Nakao; T. Inoue; Y. Kataoka; M. Kurokawa; R. A. Fissore; K. Nakamura; M. Katsuki; K. Mikoshiba; N. Yoshida; T. Takenawa

doi:10.1126/science.1059042

Requirement of phospholipase Cδ4 for the zona pellucida-induced acrosome reaction

K. Fukami^*, K. Nakao, T. Inoue, Y. Kataoka, M. Kurokawa, R. A. Fissore, K. Nakamura, M. Katsuki, K. Mikoshiba, N. Yoshida, T. Takenawa

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

174 Citations (Scopus)

Abstract

Several phospholipase C (PLC) isoforms have been found in male and female mammalian gametes, and splicing isoforms of PLCδ4 are predominantly expressed in testis. Here we report that male mice in which the PLCδ4 gene had been disrupted either produced few small litters or were sterile. In vitro fertilization studies showed that insemination with PLCδ4^-/- sperm resulted in significantly fewer eggs becoming activated and that the calcium transients associated with fertilization were absent or delayed. PLCδ4^-/- sperm were unable to initiate the acrosome reaction, an exocytotic event required for fertilization and induced by interaction with the egg coat, the zona pellucida. These data demonstrate that PLCδ4 functions in the acrosome reaction that is induced by the zona pellucida during mammalian fertilization.

Original language	English
Pages (from-to)	920-923
Number of pages	4
Journal	Science
Volume	292
Issue number	5518
DOIs	https://doi.org/10.1126/science.1059042
Publication status	Published - 2001 May 4
Externally published	Yes

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title = "Requirement of phospholipase Cδ4 for the zona pellucida-induced acrosome reaction",

abstract = "Several phospholipase C (PLC) isoforms have been found in male and female mammalian gametes, and splicing isoforms of PLCδ4 are predominantly expressed in testis. Here we report that male mice in which the PLCδ4 gene had been disrupted either produced few small litters or were sterile. In vitro fertilization studies showed that insemination with PLCδ4-/- sperm resulted in significantly fewer eggs becoming activated and that the calcium transients associated with fertilization were absent or delayed. PLCδ4-/- sperm were unable to initiate the acrosome reaction, an exocytotic event required for fertilization and induced by interaction with the egg coat, the zona pellucida. These data demonstrate that PLCδ4 functions in the acrosome reaction that is induced by the zona pellucida during mammalian fertilization.",

author = "K. Fukami and K. Nakao and T. Inoue and Y. Kataoka and M. Kurokawa and Fissore, {R. A.} and K. Nakamura and M. Katsuki and K. Mikoshiba and N. Yoshida and T. Takenawa",

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doi = "10.1126/science.1059042",

language = "English",

volume = "292",

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TY - JOUR

T1 - Requirement of phospholipase Cδ4 for the zona pellucida-induced acrosome reaction

AU - Fukami, K.

AU - Nakao, K.

AU - Inoue, T.

AU - Kataoka, Y.

AU - Kurokawa, M.

AU - Fissore, R. A.

AU - Nakamura, K.

AU - Katsuki, M.

AU - Mikoshiba, K.

AU - Yoshida, N.

AU - Takenawa, T.

PY - 2001/5/4

Y1 - 2001/5/4

N2 - Several phospholipase C (PLC) isoforms have been found in male and female mammalian gametes, and splicing isoforms of PLCδ4 are predominantly expressed in testis. Here we report that male mice in which the PLCδ4 gene had been disrupted either produced few small litters or were sterile. In vitro fertilization studies showed that insemination with PLCδ4-/- sperm resulted in significantly fewer eggs becoming activated and that the calcium transients associated with fertilization were absent or delayed. PLCδ4-/- sperm were unable to initiate the acrosome reaction, an exocytotic event required for fertilization and induced by interaction with the egg coat, the zona pellucida. These data demonstrate that PLCδ4 functions in the acrosome reaction that is induced by the zona pellucida during mammalian fertilization.

AB - Several phospholipase C (PLC) isoforms have been found in male and female mammalian gametes, and splicing isoforms of PLCδ4 are predominantly expressed in testis. Here we report that male mice in which the PLCδ4 gene had been disrupted either produced few small litters or were sterile. In vitro fertilization studies showed that insemination with PLCδ4-/- sperm resulted in significantly fewer eggs becoming activated and that the calcium transients associated with fertilization were absent or delayed. PLCδ4-/- sperm were unable to initiate the acrosome reaction, an exocytotic event required for fertilization and induced by interaction with the egg coat, the zona pellucida. These data demonstrate that PLCδ4 functions in the acrosome reaction that is induced by the zona pellucida during mammalian fertilization.

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DO - 10.1126/science.1059042

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IS - 5518

ER -

Requirement of phospholipase Cδ4 for the zona pellucida-induced acrosome reaction

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